Experiment set2S400 for Bacteroides ovatus ATCC 8483

b-Mannan borohydrate reduced carob seed 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + b-Mannan borohydrate reduced carob seed (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 14 genes in this experiment

For carbon source b-Mannan borohydrate reduced carob seed in Bacteroides ovatus ATCC 8483

For carbon source b-Mannan borohydrate reduced carob seed across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial hemoglobins	1
Chitin and N-acetylglucosamine utilization	1
Iron acquisition in Vibrio	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
Predicted carbohydrate hydrolases	1
Sialic Acid Metabolism	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Aminosugars metabolism
• Other glycan degradation
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
N-acetylneuraminate and N-acetylmannosamine degradation II	3	2	1
chitin degradation II (Vibrio)	6	3	2
β-(1,4)-mannan degradation	7	6	2
chitin degradation III (Serratia)	7	3	1
peptidoglycan recycling II	10	3	1
peptidoglycan recycling I	14	8	1