Experiment set2S399 for Bacteroides ovatus ATCC 8483

B-glucan from yeast 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + B-glucan from yeast (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 21 genes in this experiment

For carbon source B-glucan from yeast in Bacteroides ovatus ATCC 8483

For carbon source B-glucan from yeast across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	1
Lysine Biosynthesis DAP Pathway	1
Teichoic and lipoteichoic acids biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Sphingolipid metabolism
• Fructose and mannose metabolism
• Purine metabolism
• Lysine biosynthesis
• Selenoamino acid metabolism
• N-Glycan biosynthesis
• Other glycan degradation
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Sulfur metabolism
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
sulfate activation for sulfonation	2	2	1
assimilatory sulfate reduction III	3	1	1
assimilatory sulfate reduction II	3	1	1
sulfite oxidation III	3	1	1
assimilatory sulfate reduction IV	4	2	1
assimilatory sulfate reduction I	4	2	1
selenate reduction	5	2	1
Salmonella enterica serotype O:54 O antigen biosynthesis	5	2	1
dissimilatory sulfate reduction I (to hydrogen sufide))	5	1	1
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans)	6	1	1
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens)	6	1	1
L-lysine biosynthesis III	7	7	1
L-lysine biosynthesis VI	7	7	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	7	1
L-lysine biosynthesis I	9	6	1
L-lysine biosynthesis II	9	6	1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	5	1
poly(3-O-β-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis	10	2	1
poly(glycerol phosphate) wall teichoic acid biosynthesis	11	3	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	9	1
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis)	12	2	1
superpathway of sulfide oxidation (phototrophic sulfur bacteria)	12	2	1
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus)	14	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	12	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	12	1
superpathway of seleno-compound metabolism	19	5	1
aspartate superpathway	25	19	1