Experiment set2S273 for Phocaeicola dorei CL03T12C01
b-Glucan from Beet (1,2-b-Glucan); filtered 2.5 mg/ml carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + b-Glucan from Beet (1,2-b-Glucan); filtered (2.5 mg/ml)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 8 genes in this experiment
For carbon source b-Glucan from Beet (1,2-b-Glucan); filtered in Phocaeicola dorei CL03T12C01
For carbon source b-Glucan from Beet (1,2-b-Glucan); filtered across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Beta-Glucoside Metabolism | 1 |
| Fatty Acid Biosynthesis FASII | 1 |
| Iron acquisition in Vibrio | 1 |
| Ton and Tol transport systems | 1 |
| mycolic acid synthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Cyanoamino acid metabolism
- Starch and sucrose metabolism
- Phenylpropanoid biosynthesis
- Biosynthesis of unsaturated fatty acids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: