Experiment set2S216 for Escherichia coli ECRC98

WM_EV240

Group: phage
Media: Whole milk + Phage (10 MOI), pH=6.78
Culturing: Ecoli_ECRC98_ML1, 48 well microplate, Aerobic, at 25 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/18/24

Specific Phenotypes

For 30 genes in this experiment

For phage Phage in Escherichia coli ECRC98

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Campylobacter Iron Metabolism	1
DNA-replication	1
Deoxyribose and Deoxynucleoside Catabolism	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
High affinity phosphate transporter and control of PHO regulon	1
Iron acquisition in Vibrio	1
Lipid A modifications	1
Orphan regulatory proteins	1
Peptidoglycan Biosynthesis	1
Phosphate metabolism	1
Purine conversions	1
Ton and Tol transport systems	1
Transcription factors bacterial	1
Transport of Iron	1
Type IV pilus	1
cAMP signaling in bacteria	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Puromycin biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Pyrimidine metabolism
• Methionine metabolism
• Lysine degradation
• Tryptophan metabolism
• Nucleotide sugars metabolism
• Glycerolipid metabolism
• Glycerophospholipid metabolism
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Biotin metabolism
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of siderophore group nonribosomal peptides

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
guanine and guanosine salvage I	2	2	1
pseudouridine degradation	2	2	1
guanine and guanosine salvage II	2	2	1
xanthine and xanthosine salvage	2	2	1
adenine salvage	3	3	1
superpathway of guanine and guanosine salvage	3	3	1
oleate biosynthesis III (cyanobacteria)	3	2	1
adenine and adenosine salvage III	4	4	1
CDP-diacylglycerol biosynthesis II	4	4	1
CDP-diacylglycerol biosynthesis I	4	4	1
arsenic detoxification (bacteria)	4	4	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	6	3
peptidoglycan recycling II	10	7	2
phosphatidate biosynthesis (yeast)	5	3	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	2
phosphatidylglycerol biosynthesis I	6	6	1
phosphatidylglycerol biosynthesis II	6	6	1
polymyxin resistance	6	5	1
arsenate detoxification I	6	4	1
palmitoyl ethanolamide biosynthesis	6	2	1
superpathway of stearidonate biosynthesis (cyanobacteria)	6	2	1
peptidoglycan recycling I	14	14	2
diacylglycerol and triacylglycerol biosynthesis	7	3	1
stigma estolide biosynthesis	7	2	1
anandamide biosynthesis II	8	2	1
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
anandamide biosynthesis I	12	4	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of phospholipid biosynthesis II (plants)	28	10	2
peptidoglycan biosynthesis III (mycobacteria)	15	11	1