Experiment set2S145 for Escherichia coli ECRC101

D-Glucose

Group: carbon source
Media: M9_plus_D-Glucose + D-Glucose (20 mM)
Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/24

Specific Phenotypes

For 11 genes in this experiment

For carbon source D-Glucose in Escherichia coli ECRC101

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	2
Cysteine Biosynthesis	2
Methionine Biosynthesis	2
NAD and NADP cofactor biosynthesis global	2
NAD regulation	2
Fermentations: Mixed acid	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Alanine and aspartate metabolism
• Methionine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• beta-Alanine metabolism
• Selenoamino acid metabolism
• Pyruvate metabolism
• Naphthalene and anthracene degradation
• Carbon fixation in photosynthetic organisms
• Reductive carboxylate cycle (CO2 fixation)
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Sulfur metabolism
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	2
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
sulfate activation for sulfonation	2	2	1
assimilatory sulfate reduction III	3	3	1
5-(methoxycarbonylmethoxy)uridine biosynthesis	3	3	1
NAD de novo biosynthesis IV (anaerobic)	6	5	2
NAD de novo biosynthesis I	6	5	2
S-adenosyl-L-methionine salvage II	3	2	1
sulfite oxidation III	3	1	1
assimilatory sulfate reduction II	3	1	1
S-adenosyl-L-methionine salvage I	4	4	1
assimilatory sulfate reduction I	4	4	1
NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde	4	3	1
assimilatory sulfate reduction IV	4	3	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
L-methionine biosynthesis III	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
nicotine biosynthesis	9	3	2
L-methionine biosynthesis I	5	5	1
seleno-amino acid biosynthesis (plants)	5	3	1
superpathway of coenzyme A biosynthesis II (plants)	10	5	2
selenate reduction	5	2	1
dissimilatory sulfate reduction I (to hydrogen sufide))	5	1	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	10	2
L-methionine biosynthesis II	6	5	1
NAD de novo biosynthesis III	6	4	1
superpathway of nicotine biosynthesis	12	4	2
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans)	6	1	1
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens)	6	1	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
superpathway of L-homoserine and L-methionine biosynthesis	8	8	1
partial TCA cycle (obligate autotrophs)	8	7	1
nitrogen remobilization from senescing leaves	8	5	1
aspartate superpathway	25	24	3
superpathway of S-adenosyl-L-methionine biosynthesis	9	9	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	9	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
NAD de novo biosynthesis II (from tryptophan)	9	3	1
superpathway of seleno-compound metabolism	19	6	2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	6	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	8	1
superpathway of sulfide oxidation (phototrophic sulfur bacteria)	12	2	1
formaldehyde assimilation I (serine pathway)	13	8	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
superpathway of NAD biosynthesis in eukaryotes	14	7	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
mixed acid fermentation	16	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
ethene biosynthesis V (engineered)	25	18	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	22	1