Experiment set2S122 for Escherichia coli ECRC101

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Sodium-DL-Lactate

Group: carbon source
Media: M9_plus_Sodium-DL-Lactate + Sodium-DL-Lactate (20 mM)
Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/24

Specific Phenotypes

For 15 genes in this experiment

For carbon source Sodium-DL-Lactate in Escherichia coli ECRC101

For carbon source Sodium-DL-Lactate across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 2
Lactate utilization 2
Methionine Biosynthesis 2
Pyruvate metabolism I: anaplerotic reactions, PEP 2
CBSS-562.2.peg.5158 SK3 including 1
Chorismate Synthesis 1
Coenzyme A Biosynthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
D-Galacturonate and D-Glucuronate Utilization 1
D-ribose utilization 1
Fermentations: Mixed acid 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Serine-glyoxylate cycle 1
TCA Cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
3-dehydroquinate biosynthesis I 2 2 1
sulfate activation for sulfonation 2 2 1
malate/L-aspartate shuttle pathway 2 2 1
assimilatory sulfate reduction III 3 3 1
S-adenosyl-L-methionine salvage II 3 2 1
assimilatory sulfate reduction II 3 1 1
sulfite oxidation III 3 1 1
assimilatory sulfate reduction I 4 4 1
phosphopantothenate biosynthesis I 4 4 1
S-adenosyl-L-methionine salvage I 4 4 1
assimilatory sulfate reduction IV 4 3 1
L-methionine biosynthesis III 4 2 1
L-methionine biosynthesis I 5 5 1
seleno-amino acid biosynthesis (plants) 5 3 1
selenate reduction 5 2 1
dissimilatory sulfate reduction I (to hydrogen sufide)) 5 1 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 8 2
glyoxylate cycle 6 6 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 10 2
TCA cycle VIII (Chlamydia) 6 5 1
L-methionine biosynthesis II 6 5 1
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) 6 1 1
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) 6 1 1
gluconeogenesis I 13 13 2
formaldehyde assimilation I (serine pathway) 13 8 2
chorismate biosynthesis I 7 7 1
incomplete reductive TCA cycle 7 5 1
pyruvate fermentation to propanoate I 7 5 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
mixed acid fermentation 16 16 2
superpathway of L-homoserine and L-methionine biosynthesis 8 8 1
partial TCA cycle (obligate autotrophs) 8 7 1
nitrogen remobilization from senescing leaves 8 5 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 9 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 9 1
superpathway of coenzyme A biosynthesis I (bacteria) 9 9 1
TCA cycle II (plants and fungi) 9 7 1
TCA cycle IV (2-oxoglutarate decarboxylase) 9 7 1
TCA cycle V (2-oxoglutarate synthase) 9 7 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 2
superpathway of seleno-compound metabolism 19 6 2
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
TCA cycle I (prokaryotic) 10 9 1
TCA cycle III (animals) 10 8 1
glycolysis V (Pyrococcus) 10 7 1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 6 1
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
glycolysis II (from fructose 6-phosphate) 11 11 1
reductive TCA cycle I 11 8 1
L-glutamate degradation VIII (to propanoate) 11 7 1
superpathway of glyoxylate bypass and TCA 12 11 1
gluconeogenesis III 12 9 1
reductive TCA cycle II 12 8 1
superpathway of sulfide oxidation (phototrophic sulfur bacteria) 12 2 1
superpathway of L-tryptophan biosynthesis 13 13 1
glycolysis I (from glucose 6-phosphate) 13 13 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 2
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 17 1
superpathway of anaerobic energy metabolism (invertebrates) 17 12 1
superpathway of hexitol degradation (bacteria) 18 18 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 18 1
superpathway of aromatic amino acid biosynthesis 18 18 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 22 3
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 1
superpathway of anaerobic sucrose degradation 19 17 1
methylaspartate cycle 19 11 1
superpathway of N-acetylneuraminate degradation 22 22 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 1
aspartate superpathway 25 24 1
ethene biosynthesis V (engineered) 25 18 1
superpathway of chorismate metabolism 59 54 1