Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
| Pathway | #Steps | #Present | #Specific |
| CO2 fixation into oxaloacetate (anaplerotic) | 2 | 2 | 1 |
| 3-dehydroquinate biosynthesis I | 2 | 2 | 1 |
| sulfate activation for sulfonation | 2 | 2 | 1 |
| malate/L-aspartate shuttle pathway | 2 | 2 | 1 |
| assimilatory sulfate reduction III | 3 | 3 | 1 |
| S-adenosyl-L-methionine salvage II | 3 | 2 | 1 |
| assimilatory sulfate reduction II | 3 | 1 | 1 |
| sulfite oxidation III | 3 | 1 | 1 |
| assimilatory sulfate reduction I | 4 | 4 | 1 |
| phosphopantothenate biosynthesis I | 4 | 4 | 1 |
| S-adenosyl-L-methionine salvage I | 4 | 4 | 1 |
| assimilatory sulfate reduction IV | 4 | 3 | 1 |
| L-methionine biosynthesis III | 4 | 2 | 1 |
| L-methionine biosynthesis I | 5 | 5 | 1 |
| seleno-amino acid biosynthesis (plants) | 5 | 3 | 1 |
| selenate reduction | 5 | 2 | 1 |
| dissimilatory sulfate reduction I (to hydrogen sufide)) | 5 | 1 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 8 | 2 |
| glyoxylate cycle | 6 | 6 | 1 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 10 | 2 |
| TCA cycle VIII (Chlamydia) | 6 | 5 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 1 |
| superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) | 6 | 1 | 1 |
| superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) | 6 | 1 | 1 |
| gluconeogenesis I | 13 | 13 | 2 |
| formaldehyde assimilation I (serine pathway) | 13 | 8 | 2 |
| chorismate biosynthesis I | 7 | 7 | 1 |
| incomplete reductive TCA cycle | 7 | 5 | 1 |
| pyruvate fermentation to propanoate I | 7 | 5 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 1 |
| C4 photosynthetic carbon assimilation cycle, NADP-ME type | 7 | 4 | 1 |
| mixed acid fermentation | 16 | 16 | 2 |
| superpathway of L-homoserine and L-methionine biosynthesis | 8 | 8 | 1 |
| partial TCA cycle (obligate autotrophs) | 8 | 7 | 1 |
| nitrogen remobilization from senescing leaves | 8 | 5 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| superpathway of L-methionine biosynthesis (transsulfuration) | 9 | 9 | 1 |
| superpathway of S-adenosyl-L-methionine biosynthesis | 9 | 9 | 1 |
| superpathway of coenzyme A biosynthesis I (bacteria) | 9 | 9 | 1 |
| TCA cycle II (plants and fungi) | 9 | 7 | 1 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 7 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 7 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 9 | 2 |
| superpathway of seleno-compound metabolism | 19 | 6 | 2 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 1 |
| TCA cycle III (animals) | 10 | 8 | 1 |
| glycolysis V (Pyrococcus) | 10 | 7 | 1 |
| superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) | 10 | 6 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 5 | 1 |
| glycolysis II (from fructose 6-phosphate) | 11 | 11 | 1 |
| reductive TCA cycle I | 11 | 8 | 1 |
| L-glutamate degradation VIII (to propanoate) | 11 | 7 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
| gluconeogenesis III | 12 | 9 | 1 |
| reductive TCA cycle II | 12 | 8 | 1 |
| superpathway of sulfide oxidation (phototrophic sulfur bacteria) | 12 | 2 | 1 |
| superpathway of L-tryptophan biosynthesis | 13 | 13 | 1 |
| glycolysis I (from glucose 6-phosphate) | 13 | 13 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 25 | 2 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 10 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 1 |
| C4 photosynthetic carbon assimilation cycle, PEPCK type | 14 | 9 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 17 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 12 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 18 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 18 | 1 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 22 | 3 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 19 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 17 | 1 |
| methylaspartate cycle | 19 | 11 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 22 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 1 |
| aspartate superpathway | 25 | 24 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 1 |
| superpathway of chorismate metabolism | 59 | 54 | 1 |