Experiment set2IT094 for Escherichia coli BW25113

D-Glucose carbon source MOPS

Group: carbon source
Media: MOPS minimal media_noCarbon + D-Glucose (22 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 6.9 generations
By: Kelly on 6/30/2014
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 5 genes in this experiment

For carbon source D-Glucose in Escherichia coli BW25113

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	2
Arginine Biosynthesis extended	1
Lysine Biosynthesis DAP Pathway	1
Pentose phosphate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lysine biosynthesis
• Arginine and proline metabolism
• Pantothenate and CoA biosynthesis
• Pentose phosphate pathway
• Urea cycle and metabolism of amino groups
• beta-Alanine metabolism
• Glutathione metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	2
pentose phosphate pathway (oxidative branch) I	3	3	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
L-arginine degradation II (AST pathway)	5	5	1
L-ornithine biosynthesis I	5	5	1
superpathway of coenzyme A biosynthesis II (plants)	10	5	2
pentose phosphate pathway	8	8	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-lysine biosynthesis I	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of glucose and xylose degradation	17	17	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
aspartate superpathway	25	24	1