Experiment set2IT093 for Escherichia coli BW25113

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D-Galacturonic Acid monohydrate carbon source MOPS

200 most detrimental genes:

  gene name fitness t score description  
b2741 rpoS +1.9 9.4 RNA polymerase sigma factor (NCBI) compare
b4443 gcvB +1.7 2.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b3341 rpsG +1.7 2.2 30S ribosomal protein S7 (NCBI) compare
b0018 mokC +1.3 1.6 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1148 ymfM +1.3 2.6 e14 prophage; predicted protein (NCBI) compare
b3238 yhcN +1.3 2.9 orf, hypothetical protein (VIMSS) compare
b4203 rplI +1.3 4.2 50S ribosomal protein L9 (NCBI) compare
b2904 gcvH +1.2 1.8 glycine cleavage system protein H (NCBI) compare
b4578 insP +1.1 1.6 no description compare
b3979 thrT +1.1 1.5 tRNA-Thr (NCBI) compare
b2229 b2229 +1.1 3.3 orf, hypothetical protein (VIMSS) compare
b4406 yaeP +1.1 1.6 hypothetical protein (NCBI) compare
b1085 b1085 +1.1 2.8 orf, hypothetical protein (VIMSS) compare
b1550 b1550 +1.0 1.8 orf, hypothetical protein (VIMSS) compare
b0240 crl +1.0 6.4 DNA-binding transcriptional regulator (NCBI) compare
b4124 dcuR +1.0 7.9 DNA-binding response regulator in two-component regulatory system with DcuS (NCBI) compare
b0870 ltaE +1.0 3.4 L-allo-threonine aldolase, PLP-dependent (NCBI) compare
b4172 hfq +1.0 2.3 RNA-binding protein Hfq (NCBI) compare
b2587 kgtP +0.9 4.6 alpha-ketoglutarate transporter (NCBI) compare
b1134 ymfB +0.9 1.7 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b2582 trxC +0.9 2.2 thioredoxin 2 (NCBI) compare
b3929 rraA +0.9 5.8 ribonuclease activity regulator protein RraA (NCBI) compare
b1296 ycjJ +0.9 2.6 putative amino acid/amine transport protein (VIMSS) compare
b1827 kdgR +0.9 2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b4174 hflK +0.9 6.6 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1540 ydfH +0.9 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b3528 dctA +0.9 6.9 C4 dicarboxylate/orotate:H+ symporter (DctA) (from data) compare
b3752 rbsK +0.9 6.3 ribokinase (NCBI) compare
b1758 b1758 +0.9 1.5 putative cytochrome oxidase (VIMSS) compare
b2317 dedA +0.9 3.2 conserved inner membrane protein (NCBI) compare
b3769 ilvM +0.9 2.5 acetolactate synthase II, small subunit (NCBI) compare
b0960 b0960 +0.8 6.6 orf, hypothetical protein (VIMSS) compare
b2809 b2809 +0.8 2.1 orf, hypothetical protein (VIMSS) compare
b3545 proK +0.8 2.5 tRNA-Pro (NCBI) compare
b3973 birA +0.8 1.6 biotin--protein ligase (NCBI) compare
b1095 fabF +0.8 4.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1613 manA +0.8 2.1 mannose-6-phosphate isomerase (VIMSS) compare
b4163 glyV +0.8 1.6 tRNA-Gly (NCBI) compare
b4125 dcuS +0.8 5.7 sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration (NCBI) compare
b0503 ybbB +0.8 2.0 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b1557 cspB +0.8 2.4 Qin prophage; cold shock protein (NCBI) compare
b4642 yoeD +0.8 1.5 no description compare
b1511 ydeV +0.8 3.1 putative kinase (VIMSS) compare
b4515 ybgT +0.8 1.5 hypothetical protein (NCBI) compare
b2618 b2618 +0.8 2.3 orf, hypothetical protein (VIMSS) compare
b4505 insX +0.8 1.4 predicted IS protein (RefSeq) compare
b2951 yggS +0.7 2.8 predicted enzyme (NCBI) compare
b4066 yjcF +0.7 2.4 hypothetical protein (NCBI) compare
b4175 hflC +0.7 5.1 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1620 malI +0.7 1.9 DNA-binding transcriptional repressor (NCBI) compare
b4701 sokX +0.7 2.3 sok-related sRNA, function unknown (RefSeq) compare
b1351 racC +0.7 2.4 Rac prophage; predicted protein (NCBI) compare
b0955 ycbZ +0.7 4.0 predicted peptidase (NCBI) compare
b2175 spr +0.7 2.4 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b2962 yggX +0.7 1.8 hypothetical protein (NCBI) compare
b1412 azoR +0.7 1.3 acyl carrier protein phosphodiesterase (NCBI) compare
b2377 yfdY +0.7 1.4 predicted inner membrane protein (NCBI) compare
b4511 ybdZ +0.7 1.2 hypothetical protein (NCBI) compare
b4704 arrS +0.7 1.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b4391 yjjK +0.7 4.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0652 gltL +0.7 2.6 glutamate and aspartate transporter subunit (NCBI) compare
b2410 yfeH +0.7 2.3 predicted inner membrane protein (NCBI) compare
b0340 cynS +0.7 2.0 cyanate hydratase (NCBI) compare
b4450 arcZ +0.7 1.9 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b0615 citF +0.7 2.2 citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI) compare
b1524 yneH +0.7 0.8 predicted glutaminase (NCBI) compare
b3977 tyrU +0.6 1.0 tRNA-Tyr (NCBI) compare
b1278 pgpB +0.6 1.4 phosphatidylglycerophosphatase B (NCBI) compare
b1639 ydhA +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b4457 csrC +0.6 1.8 regulatory RNA (NCBI) compare
b3034 nudF +0.6 2.9 ADP-ribose pyrophosphatase (NCBI) compare
b2704 srlB +0.6 1.8 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b3742 mioC +0.6 2.5 flavodoxin (NCBI) compare
b3107 yhaL +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b4071 nrfB +0.6 1.8 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1248 b1248 +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b2807 ygdD +0.6 2.3 conserved inner membrane protein (NCBI) compare
b1743 spy +0.6 1.6 envelope stress induced periplasmic protein (NCBI) compare
b3149 yraO +0.6 2.6 DnaA initiator-associating factor for replication initiation (NCBI) compare
b0798 ybiA +0.6 1.0 hypothetical protein (NCBI) compare
b3632 rfaQ +0.6 5.8 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1514 lsrC +0.6 1.1 AI2 transporter (NCBI) compare
b0685 ybfE +0.6 1.5 orf, hypothetical protein (VIMSS) compare
b2426 ucpA +0.6 1.3 putative oxidoreductase (VIMSS) compare
b4431 rprA +0.6 1.7 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1151 ymfO +0.6 1.2 e14 prophage; conserved protein (RefSeq) compare
b2490 b2490 +0.6 2.3 putative protein processing element (VIMSS) compare
b0368 tauD +0.6 2.1 taurine dioxygenase (NCBI) compare
b2897 ygfY +0.6 1.6 hypothetical protein (NCBI) compare
b1302 puuE +0.6 1.8 GABA aminotransferase, PLP-dependent (NCBI) compare
b4176 yjeT +0.6 1.6 conserved inner membrane protein (NCBI) compare
b1171 b1171 +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b0447 ybaO +0.6 2.5 putative LRP-like transcriptional regulator (VIMSS) compare
b2204 napH +0.6 2.2 quinol dehydrogenase membrane component (NCBI) compare
b1359 ydaU +0.6 1.8 Rac prophage; conserved protein (NCBI) compare
b1427 rimL +0.6 1.4 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1293 sapB +0.6 1.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4413 sokC +0.6 1.1 regulatory antisense RNA (NCBI) compare
b0382 yaiB +0.6 4.4 hypothetical protein (NCBI) compare
b0961 yccF +0.6 1.8 conserved inner membrane protein (NCBI) compare
b1824 yobF +0.6 1.6 hypothetical protein (NCBI) compare
b4113 basR +0.6 3.0 DNA-binding response regulator in two-component regulatory system with BasS (NCBI) compare
b3095 yqjA +0.6 3.9 conserved inner membrane protein (NCBI) compare
b4146 yjeK +0.6 1.9 predicted lysine aminomutase (NCBI) compare
b1671 ydhX +0.6 0.8 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b1185 dsbB +0.6 1.1 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b2808 gcvA +0.6 2.7 DNA-binding transcriptional dual regulator (NCBI) compare
b0051 ksgA +0.6 2.9 dimethyladenosine transferase (NCBI) compare
b3928 yiiU +0.6 3.0 hypothetical protein (NCBI) compare
b4527 b1354 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b1804 rnd +0.6 1.8 ribonuclease D (NCBI) compare
b1746 astD +0.5 2.5 succinylglutamic semialdehyde dehydrogenase (NCBI) compare
b1917 yecC +0.5 1.5 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3753 rbsR +0.5 3.9 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b3226 yhcK +0.5 2.6 putative FADA-type transcriptional regulator (VIMSS) compare
b1660 ydhC +0.5 2.1 predicted transporter (NCBI) compare
b1778 yeaA +0.5 1.1 methionine sulfoxide reductase B (NCBI) compare
b2009 sbmC +0.5 1.6 DNA gyrase inhibitor (NCBI) compare
b0014 dnaK +0.5 0.8 molecular chaperone DnaK (NCBI) compare
b0097 yacA +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b4545 ypdJ +0.5 1.5 no description compare
b2903 gcvP +0.5 4.0 glycine dehydrogenase (NCBI) compare
b1930 yedF +0.5 2.0 hypothetical protein (NCBI) compare
b1334 fnr +0.5 1.8 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1948 fliP +0.5 2.3 flagellar biosynthesis protein P (NCBI) compare
b2811 ygdK +0.5 1.9 predicted Fe-S metabolism protein (NCBI) compare
b2448 yffQ +0.5 1.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b4582 yoeA +0.5 0.9 no description compare
b3198 kdsC +0.5 1.3 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2944 sprT +0.5 2.1 hypothetical protein (NCBI) compare
b4458 oxyS +0.5 0.7 global regulatory RNA (NCBI) compare
b1465 narV +0.5 0.8 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1816 yoaE +0.5 2.1 fused predicted membrane protein/conserved protein (NCBI) compare
b0246 yafW +0.5 2.1 CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b0549 ybcO +0.5 1.1 DLP12 prophage; predicted protein (NCBI) compare
b2783 chpR +0.5 1.3 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b4194 ulaB +0.5 1.6 L-ascorbate-specific enzyme IIB component of PTS (NCBI) compare
b1623 add +0.5 1.4 adenosine deaminase (NCBI) compare
b0831 yliC +0.5 1.9 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0881 clpS +0.5 1.1 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b3148 yraN +0.5 1.4 hypothetical protein (NCBI) compare
b4504 ykfH +0.5 1.5 hypothetical protein (NCBI) compare
b4444 rygA +0.5 1.5 unknown RNA (NCBI) compare
b1438 ydcQ +0.5 1.4 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4484 cpxP +0.5 2.3 periplasmic protein combats stress (NCBI) compare
b1590 ynfH +0.5 1.7 oxidoreductase, membrane subunit (NCBI) compare
b1565 ydfV +0.5 1.6 Qin prophage; predicted protein (NCBI) compare
b1661 cfa +0.5 1.8 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b4596 yciZ +0.5 0.9 hypothetical protein (RefSeq) compare
b3210 arcB +0.5 3.1 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b1001 yccE +0.5 1.1 hypothetical protein (NCBI) compare
b0967 yccW +0.5 2.3 predicted methyltransferase (RefSeq) compare
b4360 yjjA +0.5 1.5 putative glycoprotein/receptor (VIMSS) compare
b3627 rfaI +0.5 4.0 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3616 tdh +0.5 2.5 L-threonine 3-dehydrogenase (NCBI) compare
b1672 ydhW +0.5 1.3 hypothetical protein (NCBI) compare
b1602 pntB +0.5 2.1 pyridine nucleotide transhydrogenase (NCBI) compare
b2325 yfcL +0.5 1.5 hypothetical protein (NCBI) compare
b2745 truD +0.5 2.7 tRNA pseudouridine synthase D (NCBI) compare
b2798 ygdG +0.5 1.8 Ssb-binding protein, misidentified as ExoIX (RefSeq) compare
b2156 lysP +0.5 3.2 lysine transporter (NCBI) compare
b1038 csgF +0.5 1.4 predicted transport protein (NCBI) compare
b1954 dsrA +0.5 1.2 regulatory antisense RNA (NCBI) compare
b2948 yqgE +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b0643 ybeL +0.5 2.0 hypothetical protein (NCBI) compare
b2895 fldB +0.5 1.0 flavodoxin 2 (NCBI) compare
b1777 b1777 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b2123 yehR +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b2614 grpE +0.5 0.8 heat shock protein (NCBI) compare
b4622 ytcA +0.4 1.7 hypothetical protein (RefSeq) compare
b1686 ydiI +0.4 1.0 hypothetical protein (NCBI) compare
b1765 ydjA +0.4 0.5 predicted oxidoreductase (NCBI) compare
b0712 ybgK +0.4 1.9 predicted enzyme subunit (NCBI) compare
b1673 ydhV +0.4 2.2 predicted oxidoreductase (NCBI) compare
b2729 hypD +0.4 2.7 protein required for maturation of hydrogenases (NCBI) compare
b2934 cmtB +0.4 1.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2829 ptsP +0.4 3.4 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3496 yhiP +0.4 2.7 predicted transporter (NCBI) compare
b0059 hepA +0.4 3.7 ATP-dependent helicase HepA (NCBI) compare
b4630 ykgP +0.4 0.9 no description compare
b2896 ygfX +0.4 1.9 hypothetical protein (NCBI) compare
b2198 ccmD +0.4 1.0 cytochrome c biogenesis protein (NCBI) compare
b1971 yedY +0.4 1.8 hypothetical protein (NCBI) compare
b2643 yfjX +0.4 1.0 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b0366 tauB +0.4 2.2 taurine transporter subunit (NCBI) compare
b2058 wcaB +0.4 0.6 predicted acyl transferase (NCBI) compare
b2199 ccmC +0.4 1.1 heme exporter subunit (NCBI) compare
b4337 yjiO +0.4 1.9 multidrug efflux system protein (NCBI) compare
b1640 ydhH +0.4 1.3 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b1007 b1007 +0.4 0.6 orf, hypothetical protein (VIMSS) compare
b2314 dedD +0.4 1.3 putative lipoprotein (VIMSS) compare
b0705 ybfL +0.4 1.4 putative receptor protein (VIMSS) compare
b1709 btuD +0.4 0.6 vitamin B12-transporter ATPase (NCBI) compare
b3529 yhjK +0.4 3.4 predicted diguanylate cyclase (RefSeq) compare
b1823 cspC +0.4 1.1 stress protein, member of the CspA-family (NCBI) compare
b3537 yhjT +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b4675 yoaJ +0.4 1.4 expressed protein (RefSeq) compare
b0082 mraW +0.4 1.4 S-adenosyl-methyltransferase (NCBI) compare
b1251 yciI +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b4201 priB +0.4 1.1 primosomal replication protein N (NCBI) compare


Specific Phenotypes

For 12 genes in this experiment

For carbon source D-Galacturonic Acid monohydrate in Escherichia coli BW25113

For carbon source D-Galacturonic Acid monohydrate across organisms