Experiment set2IT089 for Pseudomonas fluorescens GW456-L13

LB with Aluminum chloride hydrate 2.5 mM

Group: stress
Media: LB + Aluminum chloride hydrate (2.5 mM)
Culturing: pseudo13_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/11/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1191 B5,B6

Specific Phenotypes

For 10 genes in this experiment

For stress Aluminum chloride hydrate in Pseudomonas fluorescens GW456-L13

For stress Aluminum chloride hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
Aromatic amino acid degradation	1
Chorismate Synthesis	1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tryptophan metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Methane metabolism
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-dehydroquinate biosynthesis I	2	2	1
superoxide radicals degradation	2	2	1
methanol oxidation to formaldehyde IV	2	1	1
ethanol degradation IV	3	3	1
reactive oxygen species degradation	4	4	1
chorismate biosynthesis I	7	7	1
superpathway of L-phenylalanine biosynthesis	10	10	1
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-tryptophan biosynthesis	13	13	1
superpathway of aromatic amino acid biosynthesis	18	18	1
superpathway of chorismate metabolism	59	42	1