Experiment set2IT088 for Escherichia coli BW25113

LB with 1-ethyl-3-methylimidazolium chloride 80 mM

Group: stress
Media: LB + 1-ethyl-3-methylimidazolium chloride (80 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 963 E1,E2

Specific Phenotypes

For 29 genes in this experiment

For stress 1-ethyl-3-methylimidazolium chloride in Escherichia coli BW25113

For stress 1-ethyl-3-methylimidazolium chloride across organisms

SEED Subsystems

Subsystem	#Specific
Selenocysteine metabolism	3
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	2
Beta-Glucoside Metabolism	1
CBSS-562.2.peg.5158 SK3 including	1
DNA repair, bacterial	1
Deoxyribose and Deoxynucleoside Catabolism	1
Formate dehydrogenase	1
Formate hydrogenase	1
Multidrug Resistance Efflux Pumps	1
Polyamine Metabolism	1
Protein degradation	1
Transport of Manganese	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Selenoamino acid metabolism
• Glycerophospholipid metabolism
• Urea cycle and metabolism of amino groups
• Pyrimidine metabolism
• Arginine and proline metabolism
• Aminophosphonate metabolism
• Cyanoamino acid metabolism
• Starch and sucrose metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Glycerolipid metabolism
• Sphingolipid metabolism
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Porphyrin and chlorophyll metabolism
• Phenylpropanoid biosynthesis
• Aminoacyl-tRNA biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate oxidation to CO2	1	1	1
cardiolipin biosynthesis I	3	3	2
L-selenocysteine biosynthesis I (bacteria)	3	3	2
cardiolipin biosynthesis II	3	3	2
neolinustatin bioactivation	3	2	2
siroheme biosynthesis	4	4	2
nitrate reduction III (dissimilatory)	2	2	1
formate to trimethylamine N-oxide electron transfer	2	2	1
pseudouridine degradation	2	2	1
formate to dimethyl sulfoxide electron transfer	2	2	1
linustatin bioactivation	4	2	2
linamarin degradation	2	1	1
lotaustralin degradation	2	1	1
spermine and spermidine degradation I	5	2	2
cardiolipin biosynthesis III	3	3	1
formate to nitrite electron transfer	3	2	1
cellulose degradation II (fungi)	3	2	1
factor 430 biosynthesis	7	3	2
heme b biosynthesis V (aerobic)	4	4	1
heme b biosynthesis I (aerobic)	4	4	1
heme b biosynthesis II (oxygen-independent)	4	4	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
oxalate degradation VI	4	2	1
L-selenocysteine biosynthesis II (archaea and eukaryotes)	4	2	1
putrescine degradation III	4	2	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	3
coumarin biosynthesis (via 2-coumarate)	5	2	1
oxalate degradation III	5	2	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	2
phosphatidylglycerol biosynthesis I	6	6	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	6	1
phosphatidylglycerol biosynthesis II	6	6	1
α-tomatine degradation	6	1	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
superpathway of heme b biosynthesis from glycine	8	7	1
superpathway of heme b biosynthesis from glutamate	10	10	1
superpathway of C1 compounds oxidation to CO2	12	4	1
firefly bioluminescence	14	2	1
purine nucleobases degradation I (anaerobic)	15	6	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
purine nucleobases degradation II (anaerobic)	24	17	1
superpathway of phospholipid biosynthesis II (plants)	28	10	1