Experiment set2IT087 for Escherichia coli BW25113

LB with 1-ethyl-3-methylimidazolium chloride 40 mM

Group: stress
Media: LB + 1-ethyl-3-methylimidazolium chloride (40 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 963 D7,D8

Specific Phenotypes

For 25 genes in this experiment

For stress 1-ethyl-3-methylimidazolium chloride in Escherichia coli BW25113

For stress 1-ethyl-3-methylimidazolium chloride across organisms

SEED Subsystems

Subsystem	#Specific
Formate hydrogenase	4
Selenocysteine metabolism	3
Formate dehydrogenase	2
Deoxyribose and Deoxynucleoside Catabolism	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Glycine and Serine Utilization	1
Polyamine Metabolism	1
Protein degradation	1
Pyruvate Alanine Serine Interconversions	1
Threonine anaerobic catabolism gene cluster	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Selenoamino acid metabolism
• Urea cycle and metabolism of amino groups
• Pyrimidine metabolism
• Arginine and proline metabolism
• Glycerolipid metabolism
• Glycerophospholipid metabolism
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Aminoacyl-tRNA biosynthesis
• Drug metabolism - other enzymes

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate oxidation to CO2	1	1	1
L-selenocysteine biosynthesis I (bacteria)	3	3	2
pyrimidine ribonucleosides salvage I	3	3	2
formate to trimethylamine N-oxide electron transfer	2	2	1
formate to dimethyl sulfoxide electron transfer	2	2	1
nitrate reduction III (dissimilatory)	2	2	1
spermine and spermidine degradation I	5	2	2
cardiolipin biosynthesis I	3	3	1
cardiolipin biosynthesis III	3	3	1
cardiolipin biosynthesis II	3	3	1
formate to nitrite electron transfer	3	2	1
L-selenocysteine biosynthesis II (archaea and eukaryotes)	4	2	1
oxalate degradation VI	4	2	1
putrescine degradation III	4	2	1
superpathway of pyrimidine ribonucleosides salvage	10	10	2
oxalate degradation III	5	2	1
phosphatidylglycerol biosynthesis II	6	6	1
phosphatidylglycerol biosynthesis I	6	6	1
nucleoside and nucleotide degradation (archaea)	10	4	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1
superpathway of C1 compounds oxidation to CO2	12	4	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	1
purine nucleobases degradation I (anaerobic)	15	6	1
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
purine nucleobases degradation II (anaerobic)	24	17	1
superpathway of phospholipid biosynthesis II (plants)	28	10	1