Experiment set2IT085 for Escherichia coli BW25113

Compare to:

LB with 1-ethyl-3-methylimidazolium acetate 30 mM

200 most detrimental genes:

  gene name fitness t score description  
b1036 ycdZ +2.7 11.9 orf, hypothetical protein (VIMSS) compare
b3778 rep +2.2 16.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1865 nudB +2.2 2.4 dATP pyrophosphohydrolase (NCBI) compare
b3979 thrT +2.0 2.9 tRNA-Thr (NCBI) compare
b0018 mokC +1.9 2.4 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b0846 ybjK +1.9 10.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2393 nupC +1.9 10.0 nucleoside (except guanosine) transporter (NCBI) compare
b0059 hepA +1.8 14.8 ATP-dependent helicase HepA (NCBI) compare
b3387 dam +1.7 13.4 DNA adenine methylase (NCBI) compare
b3806 cyaA +1.7 10.1 adenylate cyclase (NCBI) compare
b2175 spr +1.6 6.1 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b2912 ygfA +1.5 5.2 putative ligase (VIMSS) compare
b1235 rssB +1.5 8.9 response regulator of RpoS (NCBI) compare
b1855 lpxM +1.4 4.6 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1961 dcm +1.4 2.6 DNA cytosine methylase (NCBI) compare
b2904 gcvH +1.4 3.0 glycine cleavage system protein H (NCBI) compare
b0145 dksA +1.3 3.9 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0889 lrp +1.3 3.9 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1134 ymfB +1.3 2.4 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b4000 hupA +1.3 6.5 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b2903 gcvP +1.3 10.2 glycine dehydrogenase (NCBI) compare
b1148 ymfM +1.3 2.4 e14 prophage; predicted protein (NCBI) compare
b2951 yggS +1.2 4.9 predicted enzyme (NCBI) compare
b0463 acrA +1.2 8.3 multidrug efflux system (NCBI) compare
b3169 nusA +1.2 3.5 transcription elongation factor NusA (NCBI) compare
b0462 acrB +1.2 9.9 multidrug efflux system protein (NCBI) compare
b4481 rffT +1.2 6.8 4-alpha-L-fucosyltransferase (NCBI) compare
b0015 dnaJ +1.2 8.2 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b0564 appY +1.2 1.4 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0641 rlpB +1.1 1.7 minor lipoprotein (NCBI) compare
b3181 greA +1.1 5.3 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4406 yaeP +1.1 2.1 hypothetical protein (NCBI) compare
b1349 recT +1.1 4.6 Rac prophage; recombination and repair protein (NCBI) compare
b0125 hpt +1.1 5.1 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b4370 leuQ +1.0 2.5 tRNA-Leu (NCBI) compare
b4578 insP +1.0 1.7 no description compare
b4177 purA +1.0 3.6 adenylosuccinate synthetase (NCBI) compare
b3764 yifE +1.0 4.9 hypothetical protein (NCBI) compare
b2905 gcvT +1.0 5.2 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1296 ycjJ +1.0 2.7 putative amino acid/amine transport protein (VIMSS) compare
b1550 b1550 +1.0 1.7 orf, hypothetical protein (VIMSS) compare
b3035 tolC +1.0 6.3 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1764 selD +1.0 3.4 selenophosphate synthetase (NCBI) compare
b3790 rffC +1.0 5.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b4527 b1354 +0.9 3.0 orf, hypothetical protein (VIMSS) compare
b1171 b1171 +0.9 2.2 orf, hypothetical protein (VIMSS) compare
b1511 ydeV +0.9 3.7 putative kinase (VIMSS) compare
b1263 trpD +0.9 2.0 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b3341 rpsG +0.9 1.1 30S ribosomal protein S7 (NCBI) compare
b3405 ompR +0.9 4.4 osmolarity response regulator (NCBI) compare
b0627 tatE +0.9 1.5 twin arginine translocase protein E (NCBI) compare
b4585 sroB +0.9 1.2 Novel sRNA, function unknown (RefSeq) compare
b2202 napC +0.9 3.3 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1613 manA +0.9 1.9 mannose-6-phosphate isomerase (VIMSS) compare
b0764 modB +0.9 3.2 molybdate ABC transporter permease protein (NCBI) compare
b0438 clpX +0.9 5.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1234 rssA +0.9 3.9 conserved protein (RefSeq) compare
b3391 hofQ +0.9 5.9 predicted fimbrial transporter (NCBI) compare
b2582 trxC +0.9 2.1 thioredoxin 2 (NCBI) compare
b1540 ydfH +0.8 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3261 fis +0.8 3.0 DNA-binding protein Fis (NCBI) compare
b2480 bcp +0.8 3.4 thioredoxin-dependent thiol peroxidase (NCBI) compare
b1448 yncA +0.8 1.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2114 metG +0.8 1.2 methionyl-tRNA synthetase (NCBI) compare
b1948 fliP +0.8 2.9 flagellar biosynthesis protein P (NCBI) compare
b1251 yciI +0.8 1.8 orf, hypothetical protein (VIMSS) compare
b4194 ulaB +0.8 2.0 L-ascorbate-specific enzyme IIB component of PTS (NCBI) compare
b0817 mntR +0.8 2.5 DNA-binding transcriptional regulator of mntH (NCBI) compare
b1850 eda +0.8 1.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0688 pgm +0.8 2.9 phosphoglucomutase (NCBI) compare
b2688 gshA +0.8 4.5 glutamate--cysteine ligase (NCBI) compare
b0365 tauA +0.8 3.2 taurine transport system periplasmic protein (VIMSS) compare
b1255 yciC +0.8 3.0 hypothetical protein (NCBI) compare
b0418 pgpA +0.7 3.5 phosphatidylglycerophosphatase A (NCBI) compare
b2488 hyfH +0.7 1.5 hydrogenase 4, Fe-S subunit (NCBI) compare
b1999 yeeP +0.7 2.6 putative histone (VIMSS) compare
b3791 rffA +0.7 4.9 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2895 fldB +0.7 1.6 flavodoxin 2 (NCBI) compare
b0044 fixX +0.7 2.4 predicted 4Fe-4S ferredoxin-type protein (NCBI) compare
b1584 speG +0.7 4.3 spermidine N1-acetyltransferase (NCBI) compare
b1848 yebG +0.7 1.1 conserved protein regulated by LexA (NCBI) compare
b4239 treC +0.7 3.4 trehalose-6-P hydrolase (NCBI) compare
b2807 ygdD +0.7 2.3 conserved inner membrane protein (NCBI) compare
b2481 hyfA +0.7 2.5 hydrogenase 4 Fe-S subunit (VIMSS) compare
b2198 ccmD +0.7 1.4 cytochrome c biogenesis protein (NCBI) compare
b1890 motA +0.7 2.0 flagellar motor protein MotA (NCBI) compare
b0437 clpP +0.7 3.9 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3392 yrfA +0.7 4.2 orf, hypothetical protein (VIMSS) compare
b3977 tyrU +0.7 1.0 tRNA-Tyr (NCBI) compare
b0209 yafD +0.7 3.6 hypothetical protein (NCBI) compare
b2937 speB +0.7 4.2 agmatinase (NCBI) compare
b1426 ydcH +0.7 1.1 orf, hypothetical protein (VIMSS) compare
b2947 gshB +0.7 3.0 glutathione synthetase (NCBI) compare
b3857 mobA +0.7 3.0 molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI) compare
b1669 ydhT +0.7 1.7 hypothetical protein (NCBI) compare
b1185 dsbB +0.7 1.5 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1953 b1953 +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b3916 pfkA +0.7 2.6 6-phosphofructokinase (NCBI) compare
b3892 fdoI +0.7 4.0 formate dehydrogenase-O, cytochrome b556 subunit (NCBI) compare
b0624 crcB +0.7 2.2 camphor resistance protein CrcB (NCBI) compare
b1253 yciA +0.7 1.7 predicted hydrolase (NCBI) compare
b3973 birA +0.7 1.2 biotin--protein ligase (NCBI) compare
b2698 recX +0.7 1.5 RecA regulator RecX (NCBI) compare
b0778 bioD +0.7 2.1 dethiobiotin synthetase (NCBI) compare
b1261 trpB +0.7 1.1 tryptophan synthase subunit beta (NCBI) compare
b1938 fliF +0.7 3.5 flagellar M-ring protein (NCBI) compare
b3423 glpR +0.7 4.1 DNA-binding transcriptional repressor (NCBI) compare
b3206 npr +0.6 2.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2829 ptsP +0.6 5.1 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b4431 rprA +0.6 1.7 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3404 envZ +0.6 4.8 osmolarity sensor protein (NCBI) compare
b3894 fdoG +0.6 5.5 formate dehydrogenase-O, large subunit (NCBI) compare
b1565 ydfV +0.6 2.1 Qin prophage; predicted protein (NCBI) compare
b2897 ygfY +0.6 1.7 hypothetical protein (NCBI) compare
b1427 rimL +0.6 1.1 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b0707 ybgA +0.6 1.8 hypothetical protein (NCBI) compare
b2186 yejK +0.6 4.1 nucleoid-associated protein NdpA (NCBI) compare
b0246 yafW +0.6 2.0 CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b0440 hupB +0.6 2.8 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b0658 ybeX +0.6 3.7 predicteed ion transport (NCBI) compare
b2935 tktA +0.6 1.8 transketolase 1, thiamin-binding (NCBI) compare
b3860 dsbA +0.6 4.2 periplasmic protein disulfide isomerase I (NCBI) compare
b1976 b1976 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b2540 hcaC +0.6 1.1 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI) compare
b2096 gatY +0.6 1.7 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b0393 rdgC +0.6 4.0 recombination associated protein (NCBI) compare
b1824 yobF +0.6 1.9 hypothetical protein (NCBI) compare
b0539 ybcC +0.6 2.3 DLP12 prophage; predicted exonuclease (RefSeq) compare
b0632 dacA +0.6 3.4 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3180 yhbY +0.6 2.9 predicted RNA-binding protein (NCBI) compare
b1944 fliL +0.6 2.0 flagellar basal body-associated protein FliL (NCBI) compare
b2240 glpT +0.6 3.7 sn-glycerol-3-phosphate transporter (NCBI) compare
b2708 gutQ +0.6 2.4 orf, hypothetical protein (VIMSS) compare
b1639 ydhA +0.6 1.5 orf, hypothetical protein (VIMSS) compare
b1214 ychA +0.6 2.1 predicted transcriptional regulator (NCBI) compare
b1628 rsxB +0.6 1.1 electron transport complex protein RnfB (NCBI) compare
b0505 allA +0.6 2.0 ureidoglycolate hydrolase (NCBI) compare
b1949 fliQ +0.6 1.5 flagellar biosynthesis protein Q (NCBI) compare
b1524 yneH +0.6 0.7 predicted glutaminase (NCBI) compare
b2683 ygaH +0.6 2.6 predicted inner membrane protein (NCBI) compare
b2017 yefM +0.6 0.6 orf, hypothetical protein (VIMSS) compare
b1103 ycfF +0.6 0.8 orf, hypothetical protein (VIMSS) compare
b0964 yccT +0.6 1.9 hypothetical protein (NCBI) compare
b2044 wcaL +0.6 2.1 predicted glycosyl transferase (NCBI) compare
b0142 folK +0.6 0.8 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3895 fdhD +0.6 3.6 formate dehydrogenase accessory protein (NCBI) compare
b3591 selA +0.6 4.3 selenocysteine synthase (NCBI) compare
b1097 yceG +0.6 2.8 predicted aminodeoxychorismate lyase (NCBI) compare
b2037 rfbX +0.6 4.2 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b1160 elbA +0.5 1.0 hypothetical protein (NCBI) compare
b0781 moaA +0.5 2.9 molybdenum cofactor biosynthesis protein A (NCBI) compare
b1939 fliG +0.5 1.6 flagellar motor switch protein G (NCBI) compare
b0643 ybeL +0.5 2.2 hypothetical protein (NCBI) compare
b3590 selB +0.5 4.3 selenocysteinyl-tRNA-specific translation factor (NCBI) compare
b2977 glcG +0.5 1.3 hypothetical protein (NCBI) compare
b0828 iaaA +0.5 1.9 L-asparaginase (NCBI) compare
b0368 tauD +0.5 1.8 taurine dioxygenase (NCBI) compare
b3260 dusB +0.5 2.2 tRNA-dihydrouridine synthase B (NCBI) compare
b1945 fliM +0.5 2.6 flagellar motor switch protein M (NCBI) compare
b4606 ypfM +0.5 2.2 hypothetical protein (RefSeq) compare
b4444 rygA +0.5 1.6 unknown RNA (NCBI) compare
b4480 hdfR +0.5 2.7 transcriptional regulator HdfR (NCBI) compare
b1007 b1007 +0.5 0.7 orf, hypothetical protein (VIMSS) compare
b1083 flgL +0.5 1.8 flagellar hook-associated protein L (NCBI) compare
b2291 yfbR +0.5 1.5 hypothetical protein (NCBI) compare
b1087 yceF +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b0014 dnaK +0.5 0.9 molecular chaperone DnaK (NCBI) compare
b1661 cfa +0.5 1.7 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b4001 yjaH +0.5 2.5 hypothetical protein (NCBI) compare
b3926 glpK +0.5 4.4 glycerol kinase (NCBI) compare
b4293 fecI +0.5 2.2 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b1486 ddpB +0.5 0.9 D-ala-D-ala transporter subunit (NCBI) compare
b1760 ynjH +0.5 1.3 hypothetical protein (NCBI) compare
b0841 ybjG +0.5 1.8 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b4506 ykgO +0.5 1.7 rpmJ (L36) paralog (NCBI) compare
b0785 moaE +0.5 1.8 molybdopterin synthase, large subunit (NCBI) compare
b2236 yfaE +0.5 0.9 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1344 ydaO +0.5 1.7 predicted C32 tRNA thiolase (NCBI) compare
b1431 ydcL +0.5 1.4 predicted lipoprotein (NCBI) compare
b4522 yciX_1 +0.5 1.3 hypothetical protein (NCBI) compare
b2614 grpE +0.5 0.7 heat shock protein (NCBI) compare
b4411 ecnB +0.5 1.1 entericidin B membrane lipoprotein (NCBI) compare
b0460 hha +0.5 2.7 modulator of gene expression, with H-NS (NCBI) compare
b0080 fruR +0.5 2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2170 setB +0.5 1.8 lactose/glucose efflux system (NCBI) compare
b1035 ycdY +0.5 2.2 hypothetical protein (NCBI) compare
b4592 yccB +0.5 0.8 hypothetical protein (RefSeq) compare
b4596 yciZ +0.5 0.8 hypothetical protein (RefSeq) compare
b1778 yeaA +0.5 0.6 methionine sulfoxide reductase B (NCBI) compare
b0870 ltaE +0.5 1.7 L-allo-threonine aldolase, PLP-dependent (NCBI) compare
b2783 chpR +0.5 1.1 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b3475 acpT +0.5 2.1 holo-(acyl carrier protein) synthase 2 (NCBI) compare
b2704 srlB +0.5 1.3 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b4622 ytcA +0.5 1.9 hypothetical protein (RefSeq) compare
b2004 yeeU +0.5 1.6 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1187 fadR +0.5 2.1 fatty acid metabolism regulator (NCBI) compare
b2722 hycD +0.5 2.0 hydrogenase 3, membrane subunit (NCBI) compare
b2229 b2229 +0.5 1.1 orf, hypothetical protein (VIMSS) compare
b2473 ypfH +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1514 lsrC +0.5 0.8 AI2 transporter (NCBI) compare


Specific Phenotypes

For 9 genes in this experiment

For stress 1-ethyl-3-methylimidazolium acetate in Escherichia coli BW25113

For stress 1-ethyl-3-methylimidazolium acetate across organisms