Experiment set2IT084 for Agrobacterium fabrum C58

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Betanin carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Betanin (5 mg/ml)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 10/20/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 20 genes in this experiment

For carbon source Betanin in Agrobacterium fabrum C58

For carbon source Betanin across organisms

SEED Subsystems

Subsystem #Specific
Fatty Acid Biosynthesis FASII 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycine and Serine Utilization 1
Oxidative stress 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Serine Biosynthesis 1
Xylose utilization 1
mycolic acid synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-glucarate degradation II 3 3 1
L-serine biosynthesis I 3 3 1
L-cysteine biosynthesis IX (Trichomonas vaginalis) 3 2 1
alginate degradation 7 4 2
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) 12 12 3
gondoate biosynthesis (anaerobic) 4 4 1
superpathway of L-serine and glycine biosynthesis I 4 4 1
D-glucarate degradation I 4 2 1
palmitate biosynthesis III 29 28 7
tetradecanoate biosynthesis (mitochondria) 25 23 6
palmitate biosynthesis II (type II fatty acid synthase) 31 29 7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 9 8 2
oleate biosynthesis IV (anaerobic) 14 13 3
superpathway of fatty acids biosynthesis (E. coli) 53 49 11
8-amino-7-oxononanoate biosynthesis IV 5 5 1
fatty acid elongation -- saturated 5 5 1
superpathway of unsaturated fatty acids biosynthesis (E. coli) 20 18 4
cis-vaccenate biosynthesis 5 4 1
superpathway of D-glucarate and D-galactarate degradation 5 3 1
superpathway of fatty acid biosynthesis II (plant) 43 38 8
8-amino-7-oxononanoate biosynthesis I 11 9 2
even iso-branched-chain fatty acid biosynthesis 34 30 6
odd iso-branched-chain fatty acid biosynthesis 34 30 6
anteiso-branched-chain fatty acid biosynthesis 34 30 6
(5Z)-dodecenoate biosynthesis I 6 6 1
stearate biosynthesis II (bacteria and plants) 6 5 1
(5Z)-dodecenoate biosynthesis II 6 5 1
stearate biosynthesis IV 6 4 1
petroselinate biosynthesis 6 2 1
streptorubin B biosynthesis 34 20 5
biotin biosynthesis I 15 13 2
superpathway of fatty acid biosynthesis I (E. coli) 16 15 2
2-allylmalonyl-CoA biosynthesis 8 2 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 22 1
mycolate biosynthesis 205 21 5
superpathway of mycolate biosynthesis 239 22 5