Experiment set2IT081 for Escherichia coli BW25113

Compare to:

LB with Cholin acetate 150 mM

200 most detrimental genes:

  gene name fitness t score description  
b3261 fis +2.3 8.5 DNA-binding protein Fis (NCBI) compare
b2904 gcvH +2.3 6.1 glycine cleavage system protein H (NCBI) compare
b4171 miaA +2.1 12.6 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2808 gcvA +2.1 11.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0889 lrp +2.0 6.3 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2903 gcvP +1.8 14.3 glycine dehydrogenase (NCBI) compare
b0059 hepA +1.8 15.4 ATP-dependent helicase HepA (NCBI) compare
b3181 greA +1.8 7.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1865 nudB +1.7 2.1 dATP pyrophosphohydrolase (NCBI) compare
b2905 gcvT +1.7 8.7 glycine cleavage system aminomethyltransferase T (NCBI) compare
b2830 nudH +1.5 3.5 dinucleoside polyphosphate hydrolase (NCBI) compare
b1148 ymfM +1.4 2.7 e14 prophage; predicted protein (NCBI) compare
b4260 pepA +1.4 10.4 leucyl aminopeptidase (NCBI) compare
b2951 yggS +1.3 5.1 predicted enzyme (NCBI) compare
b4443 gcvB +1.2 1.7 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b3260 dusB +1.2 4.8 tRNA-dihydrouridine synthase B (NCBI) compare
b1824 yobF +1.1 2.5 hypothetical protein (NCBI) compare
b3500 gor +1.0 1.8 glutathione reductase (NCBI) compare
b2895 fldB +1.0 2.3 flavodoxin 2 (NCBI) compare
b3412 bioH +1.0 6.0 carboxylesterase of pimeloyl-CoA synthesis (NCBI) compare
b3341 rpsG +1.0 1.6 30S ribosomal protein S7 (NCBI) compare
b1248 b1248 +1.0 1.7 orf, hypothetical protein (VIMSS) compare
b4504 ykfH +0.9 2.6 hypothetical protein (NCBI) compare
b1263 trpD +0.9 1.9 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b1671 ydhX +0.9 1.7 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b0210 yafE +0.9 2.7 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b0967 yccW +0.9 3.8 predicted methyltransferase (RefSeq) compare
b1793 yoaF +0.9 2.0 conserved outer membrane protein (NCBI) compare
b3764 yifE +0.9 4.1 hypothetical protein (NCBI) compare
b4411 ecnB +0.9 2.5 entericidin B membrane lipoprotein (NCBI) compare
b2576 srmB +0.8 2.9 ATP-dependent RNA helicase (NCBI) compare
b2915 yqfE +0.8 2.3 hypothetical protein (RefSeq) compare
b1524 yneH +0.8 1.0 predicted glutaminase (NCBI) compare
b1511 ydeV +0.8 3.1 putative kinase (VIMSS) compare
b1639 ydhA +0.8 2.2 orf, hypothetical protein (VIMSS) compare
b4402 yjjY +0.8 1.3 hypothetical protein (NCBI) compare
b3391 hofQ +0.8 6.0 predicted fimbrial transporter (NCBI) compare
b3206 npr +0.8 3.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4203 rplI +0.7 2.0 50S ribosomal protein L9 (NCBI) compare
b2476 purC +0.7 2.1 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b4371 rsmC +0.7 4.0 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b0964 yccT +0.7 2.4 hypothetical protein (NCBI) compare
b1134 ymfB +0.7 1.2 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b3281 aroE +0.7 5.8 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b4545 ypdJ +0.7 2.0 no description compare
b1693 aroD +0.7 3.4 3-dehydroquinate dehydratase (NCBI) compare
b3973 birA +0.7 1.3 biotin--protein ligase (NCBI) compare
b2093 gatB +0.7 2.1 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b1427 rimL +0.7 1.1 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b0556 rzpD +0.7 1.4 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b2618 b2618 +0.7 2.0 orf, hypothetical protein (VIMSS) compare
b2012 yeeD +0.7 1.4 hypothetical protein (NCBI) compare
b1812 pabB +0.7 2.3 para-aminobenzoate synthase component I (NCBI) compare
b1757 b1757 +0.7 2.3 putative thiosulfate sulfur transferase (VIMSS) compare
b0712 ybgK +0.7 2.9 predicted enzyme subunit (NCBI) compare
b1685 ydiH +0.7 1.0 predicted protein (RefSeq) compare
b2217 rcsB +0.7 3.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b2829 ptsP +0.7 5.0 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2198 ccmD +0.7 1.5 cytochrome c biogenesis protein (NCBI) compare
b3129 sohA +0.6 1.7 predicted regulator (NCBI) compare
b1302 puuE +0.6 1.9 GABA aminotransferase, PLP-dependent (NCBI) compare
b1566 flxA +0.6 2.0 Qin prophage; predicted protein (NCBI) compare
b4578 insP +0.6 0.9 no description compare
b1814 sdaA +0.6 2.0 L-serine deaminase I (NCBI) compare
b4413 sokC +0.6 1.1 regulatory antisense RNA (NCBI) compare
b1999 yeeP +0.6 2.2 putative histone (VIMSS) compare
b1684 sufA +0.6 0.8 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b4000 hupA +0.6 2.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0707 ybgA +0.6 1.3 hypothetical protein (NCBI) compare
b1704 aroH +0.6 2.5 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI) compare
b4176 yjeT +0.6 1.3 conserved inner membrane protein (NCBI) compare
b2506 yfgI +0.6 1.4 hypothetical protein (NCBI) compare
b3860 dsbA +0.6 4.1 periplasmic protein disulfide isomerase I (NCBI) compare
b1846 yebE +0.6 2.0 hypothetical protein (NCBI) compare
b0238 gpt +0.6 1.9 xanthine phosphoribosyltransferase (NCBI) compare
b0627 tatE +0.6 0.8 twin arginine translocase protein E (NCBI) compare
b1539 ydfG +0.6 1.6 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b4195 ulaC +0.6 1.9 L-ascorbate-specific enzyme IIA component of PTS (NCBI) compare
b1183 umuD +0.6 0.8 DNA polymerase V, subunit D (NCBI) compare
b2024 hisA +0.6 0.7 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b4505 insX +0.6 0.8 predicted IS protein (RefSeq) compare
b1843 yobB +0.6 2.0 hypothetical protein (NCBI) compare
b1955 yedP +0.6 1.0 hypothetical protein (NCBI) compare
b4515 ybgT +0.6 1.0 hypothetical protein (NCBI) compare
b2176 rtn +0.6 3.7 hypothetical protein (NCBI) compare
b1007 b1007 +0.6 0.8 orf, hypothetical protein (VIMSS) compare
b1871 yecP +0.6 2.0 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b3977 tyrU +0.6 0.8 tRNA-Tyr (NCBI) compare
b1804 rnd +0.6 1.8 ribonuclease D (NCBI) compare
b1392 paaE +0.6 1.7 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b1234 rssA +0.6 2.4 conserved protein (RefSeq) compare
b4063 soxR +0.6 2.1 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b4593 ymgI +0.6 1.5 hypothetical protein (RefSeq) compare
b4558 yifL +0.6 1.6 predicted lipoprotein (NCBI) compare
b4375 prfC +0.6 3.2 peptide chain release factor 3 (NCBI) compare
b2906 visC +0.6 2.9 hypothetical protein (NCBI) compare
b0881 clpS +0.6 1.2 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b4653 yicT +0.5 1.2 no description compare
b1296 ycjJ +0.5 1.3 putative amino acid/amine transport protein (VIMSS) compare
b1428 ydcK +0.5 2.1 predicted enzyme (NCBI) compare
b1412 azoR +0.5 0.9 acyl carrier protein phosphodiesterase (NCBI) compare
b1672 ydhW +0.5 1.6 hypothetical protein (NCBI) compare
b2597 yfiA +0.5 1.1 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1251 yciI +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b4445 omrB +0.5 1.6 sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq) compare
b2646 ypjF +0.5 1.3 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b0822 ybiV +0.5 1.7 predicted hydrolase (NCBI) compare
b2229 b2229 +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b1485 ddpC +0.5 1.7 D-ala-D-ala transporter subunit (NCBI) compare
b2426 ucpA +0.5 1.0 putative oxidoreductase (VIMSS) compare
b1290 sapF +0.5 1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2189 proL +0.5 1.1 tRNA-Pro (NCBI) compare
b4234 yjgA +0.5 1.8 hypothetical protein (NCBI) compare
b1765 ydjA +0.5 0.5 predicted oxidoreductase (NCBI) compare
b4373 rimI +0.5 1.2 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b4561 insM +0.5 1.8 transposase (RefSeq) compare
b1448 yncA +0.5 0.9 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4066 yjcF +0.5 1.4 hypothetical protein (NCBI) compare
b2329 aroC +0.5 2.2 chorismate synthase (NCBI) compare
b2811 ygdK +0.5 1.6 predicted Fe-S metabolism protein (NCBI) compare
b2291 yfbR +0.5 1.2 hypothetical protein (NCBI) compare
b0630 lipB +0.5 1.2 protein of lipoate biosynthesis (VIMSS) compare
b2214 yojL +0.5 2.1 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b1185 dsbB +0.5 1.0 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1952 dsrB +0.5 1.6 hypothetical protein (NCBI) compare
b3899 frvB +0.5 3.0 PTS system, fructose-like enzyme IIBC component (VIMSS) compare
b4480 hdfR +0.5 2.5 transcriptional regulator HdfR (NCBI) compare
b0858 ybjO +0.5 0.8 predicted inner membrane protein (NCBI) compare
b1222 narX +0.5 1.7 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b3149 yraO +0.5 2.4 DnaA initiator-associating factor for replication initiation (NCBI) compare
b4585 sroB +0.5 0.6 Novel sRNA, function unknown (RefSeq) compare
b4435 isrC +0.5 0.9 unknown RNA (NCBI) compare
b0851 nfsA +0.5 1.8 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b2770 ygcR +0.5 2.6 putative transport protein (VIMSS) compare
b2784 relA +0.5 3.1 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b1589 ynfG +0.5 1.0 oxidoreductase, Fe-S subunit (NCBI) compare
b0860 artJ +0.5 1.9 arginine transporter subunit (NCBI) compare
b1424 mdoD +0.5 1.7 glucan biosynthesis protein, periplasmic (RefSeq) compare
b3378 yhfU +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b0549 ybcO +0.5 1.0 DLP12 prophage; predicted protein (NCBI) compare
b0357 yaiN +0.5 0.9 putative alpha helix chain (VIMSS) compare
b0952 ymbA +0.5 2.1 conserved protein (RefSeq) compare
b1827 kdgR +0.5 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1742 b1742 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b1079 flgH +0.5 1.5 flagellar L-ring protein precursor H (NCBI) compare
b2853 ygeI +0.5 1.5 hypothetical protein (NCBI) compare
b1383 ydbL +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b2237 inaA +0.5 1.5 hypothetical protein (NCBI) compare
b0624 crcB +0.5 1.5 camphor resistance protein CrcB (NCBI) compare
b0660 ybeZ +0.5 1.6 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b1746 astD +0.5 1.9 succinylglutamic semialdehyde dehydrogenase (NCBI) compare
b3392 yrfA +0.4 2.6 orf, hypothetical protein (VIMSS) compare
b1266 yciV +0.4 1.1 hypothetical protein (NCBI) compare
b1679 sufE +0.4 1.5 cysteine desufuration protein SufE (NCBI) compare
b1083 flgL +0.4 1.2 flagellar hook-associated protein L (NCBI) compare
b2342 yfcY +0.4 1.9 acetyl-CoA acetyltransferase (NCBI) compare
b2003 yeeT +0.4 1.2 CP4-44 prophage; predicted protein (NCBI) compare
b1481 b1481 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3238 yhcN +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1897 otsB +0.4 1.5 trehalose-6-phosphate phosphatase, biosynthetic (NCBI) compare
b1945 fliM +0.4 2.2 flagellar motor switch protein M (NCBI) compare
b0831 yliC +0.4 1.6 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4012 yjaB +0.4 1.7 predicted acetyltransferase (NCBI) compare
b1344 ydaO +0.4 1.7 predicted C32 tRNA thiolase (NCBI) compare
b0764 modB +0.4 1.7 molybdate ABC transporter permease protein (NCBI) compare
b2839 lysR +0.4 1.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2124 yehS +0.4 2.1 hypothetical protein (NCBI) compare
b3740 gidB +0.4 2.9 glucose-inhibited division protein B (NCBI) compare
b0908 aroA +0.4 2.7 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b0368 tauD +0.4 1.4 taurine dioxygenase (NCBI) compare
b2258 b2258 +0.4 0.6 putative transport/receptor protein (VIMSS) compare
b2130 yehY +0.4 1.9 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2706 gutM +0.4 1.2 DNA-binding transcriptional activator of glucitol operon (NCBI) compare
b1778 yeaA +0.4 0.9 methionine sulfoxide reductase B (NCBI) compare
b1334 fnr +0.4 1.0 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4360 yjjA +0.4 1.2 putative glycoprotein/receptor (VIMSS) compare
b0841 ybjG +0.4 1.5 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b0977 hyaF +0.4 1.5 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b4277 yjgZ +0.4 1.6 KpLE2 phage-like element; predicted protein (NCBI) compare
b0948 ycbY +0.4 2.1 predicted methyltransferase (NCBI) compare
b0968 yccX +0.4 1.4 predicted acylphosphatase (NCBI) compare
b4633 xisD +0.4 1.3 no description compare
b2445 yffN +0.4 1.3 CPZ-55 prophage; predicted protein (RefSeq) compare
b1075 flgD +0.4 1.0 flagellar basal body rod modification protein D (NCBI) compare
b1686 ydiI +0.4 0.9 hypothetical protein (NCBI) compare
b3068 ygjF +0.4 1.1 G/U mismatch-specific DNA glycosylase (NCBI) compare
b1987 cbl +0.4 1.8 DNA-binding transcriptional activator of cysteine biosynthesis (NCBI) compare
b2004 yeeU +0.4 1.4 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b0345 lacI +0.4 2.3 transcriptional repressor of the lac operon (VIMSS) compare
b2809 b2809 +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b4330 yjiH +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b1255 yciC +0.4 1.5 hypothetical protein (NCBI) compare
b2325 yfcL +0.4 1.2 hypothetical protein (NCBI) compare
b2410 yfeH +0.4 1.3 predicted inner membrane protein (NCBI) compare
b4701 sokX +0.4 0.9 sok-related sRNA, function unknown (RefSeq) compare
b0825 mipB +0.4 1.2 putative transaldolase (VIMSS) compare
b2810 csdA +0.4 2.0 cysteine sulfinate desulfinase (NCBI) compare
b1188 ycgB +0.4 1.7 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
b4527 b1354 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b0216 aspV +0.4 1.2 tRNA-Asp (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment

For stress Cholin acetate in Escherichia coli BW25113

For stress Cholin acetate across organisms