Experiment set2IT081 for Echinicola vietnamensis KMM 6221, DSM 17526

Compare to:

L-Rhamnose monohydrate carbon source

Group: carbon source
Media: DinoMM_noCarbon_HighNutrient + L-Rhamnose monohydrate (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 3.7 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1714 B3

Specific Phenotypes

For 22 genes in this experiment

For carbon source L-Rhamnose monohydrate in Echinicola vietnamensis KMM 6221, DSM 17526

For carbon source L-Rhamnose monohydrate across organisms

SEED Subsystems

Subsystem #Specific
L-rhamnose utilization 7
Iron acquisition in Vibrio 2
Lactate utilization 2
Ton and Tol transport systems 2
Beta-Glucoside Metabolism 1
Calvin-Benson cycle 1
Chitin and N-acetylglucosamine utilization 1
Fructooligosaccharides(FOS) and Raffinose Utilization 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Mannose Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
methylglyoxal degradation V 3 3 2
neolinustatin bioactivation 3 2 2
L-rhamnose degradation I 5 5 3
linustatin bioactivation 4 2 2
lotaustralin degradation 2 1 1
linamarin degradation 2 1 1
L-lactaldehyde degradation (aerobic) 2 1 1
cellulose degradation II (fungi) 3 2 1
methylglyoxal degradation IV 3 2 1
superpathway of fucose and rhamnose degradation 12 7 4
coumarin biosynthesis (via 2-coumarate) 5 2 1
lactate biosynthesis (archaea) 5 2 1
α-tomatine degradation 6 1 1
superpathway of methylglyoxal degradation 8 5 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 2 1
L-rhamnose degradation II 8 2 1
sucrose biosynthesis I (from photosynthesis) 9 6 1
glycolysis II (from fructose 6-phosphate) 11 9 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
gluconeogenesis III 12 9 1
Calvin-Benson-Bassham cycle 13 11 1
gluconeogenesis I 13 11 1
glycolysis I (from glucose 6-phosphate) 13 10 1
firefly bioluminescence 14 2 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 13 1
oxygenic photosynthesis 17 11 1
superpathway of hexitol degradation (bacteria) 18 12 1
superpathway of anaerobic sucrose degradation 19 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 10 1
superpathway of N-acetylneuraminate degradation 22 13 1
ethene biosynthesis V (engineered) 25 18 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 19 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 1
1-butanol autotrophic biosynthesis (engineered) 27 20 1