Experiment set2IT080 for Escherichia coli BW25113

Compare to:

LB with Cholin acetate 75 mM

200 most important genes:

  gene name fitness t score description  
b0049 apaH -3.6 -5.9 diadenosinetetraphosphatase (NCBI) compare
b3962 udhA -2.9 -8.4 putative oxidoreductase (VIMSS) compare
b0226 dinJ -2.5 -4.4 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1860 ruvB -2.4 -2.2 Holliday junction DNA helicase B (NCBI) compare
b4172 hfq -2.3 -2.2 RNA-binding protein Hfq (NCBI) compare
b1658 purR -2.3 -6.8 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b2479 gcvR -2.3 -3.7 transcriptional regulation of gcv operon (VIMSS) compare
b3636 rpmG -2.2 -2.0 50S ribosomal protein L33 (NCBI) compare
b2820 recB -2.0 -1.4 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b2528 iscA -2.0 -1.6 iron-sulfur cluster assembly protein (NCBI) compare
b0603 ybdO -2.0 -3.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1279 yciS -1.9 -2.0 conserved inner membrane protein (NCBI) compare
b1677 lpp -1.9 -3.2 murein lipoprotein (NCBI) compare
b0726 sucA -1.9 -3.4 alpha-ketoglutarate decarboxylase (NCBI) compare
b3121 yhaC -1.9 -2.5 hypothetical protein (NCBI) compare
b1863 ruvC -1.9 -3.8 Holliday junction resolvase (NCBI) compare
b2699 recA -1.9 -2.1 recombinase A (NCBI) compare
b3493 pitA -1.8 -6.8 phosphate transporter, low-affinity (NCBI) compare
b0439 lon -1.8 -4.9 DNA-binding ATP-dependent protease La (NCBI) compare
b3463 ftsE -1.7 -3.7 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3792 wzxE -1.7 -7.4 O-antigen translocase (NCBI) compare
b3237 argR -1.7 -4.3 arginine repressor (NCBI) compare
b3504 yhiS -1.7 -3.4 hypothetical protein (RefSeq) compare
b2650 ypjC -1.6 -2.3 hypothetical protein (RefSeq) compare
b0627 tatE -1.6 -1.7 twin arginine translocase protein E (NCBI) compare
b0912 ihfB -1.6 -3.2 integration host factor subunit beta (NCBI) compare
b1063 yceB -1.6 -2.3 predicted lipoprotein (NCBI) compare
b3390 aroK -1.6 -2.0 shikimate kinase I (NCBI) compare
b3462 ftsX -1.6 -4.8 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2567 rnc -1.6 -2.0 ribonuclease III (NCBI) compare
b3935 priA -1.5 -2.8 primosome assembly protein PriA (NCBI) compare
b0890 ftsK -1.5 -5.9 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1257 yciE -1.5 -2.2 hypothetical protein (NCBI) compare
b2894 xerD -1.5 -1.6 site-specific tyrosine recombinase XerD (NCBI) compare
b0866 ybjQ -1.5 -1.5 hypothetical protein (NCBI) compare
b1712 ihfA -1.4 -2.9 integration host factor subunit alpha (NCBI) compare
b0782 moaB -1.4 -2.2 molybdopterin biosynthesis protein B (NCBI) compare
b1545 pinQ -1.4 -1.6 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1358 ydaT -1.4 -1.9 Rac prophage; predicted protein (NCBI) compare
b0118 acnB -1.4 -2.5 aconitate hydratase (NCBI) compare
b3021 ygiT -1.4 -3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2055 wcaE -1.4 -3.3 predicted glycosyl transferase (NCBI) compare
b1942 fliJ -1.4 -1.4 flagellar biosynthesis chaperone (NCBI) compare
b0741 pal -1.3 -3.6 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3229 sspA -1.3 -3.2 stringent starvation protein A (NCBI) compare
b0738 tolR -1.3 -2.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0080 fruR -1.3 -4.7 DNA-binding transcriptional dual regulator (NCBI) compare
b3613 yibP -1.3 -4.4 putative membrane protein (VIMSS) compare
b2518 ndk -1.3 -2.1 nucleoside diphosphate kinase (NCBI) compare
b0739 tolA -1.3 -3.4 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3911 cpxA -1.3 -7.3 two-component sensor protein (NCBI) compare
b3725 pstB -1.2 -2.1 phosphate transporter subunit (NCBI) compare
b4163 glyV -1.2 -1.1 tRNA-Gly (NCBI) compare
b0683 fur -1.2 -1.4 ferric uptake regulator (NCBI) compare
b0721 sdhC -1.2 -3.4 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b0500 ybbD -1.2 -2.1 hypothetical protein (RefSeq) compare
b2288 nuoA -1.2 -2.7 NADH dehydrogenase I chain A (VIMSS) compare
b4201 priB -1.2 -1.5 primosomal replication protein N (NCBI) compare
b0407 yajC -1.2 -2.5 preprotein translocase subunit YajC (NCBI) compare
b3290 trkA -1.2 -2.6 potassium transporter peripheral membrane component (NCBI) compare
b0740 tolB -1.2 -3.4 translocation protein TolB precursor (NCBI) compare
b1252 tonB -1.2 -2.4 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b0200 gmhB -1.2 -1.3 hypothetical protein (NCBI) compare
b0949 uup -1.2 -5.2 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3839 tatC -1.2 -6.0 TatABCE protein translocation system subunit (NCBI) compare
b1926 fliT -1.1 -2.2 predicted chaperone (NCBI) compare
b2071 yegJ -1.1 -2.2 hypothetical protein (NCBI) compare
b2569 lepA -1.1 -3.3 GTP-binding protein LepA (NCBI) compare
b0219 yafV -1.1 -2.4 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b2110 yehC -1.1 -2.8 predicted periplasmic pilin chaperone (NCBI) compare
b1642 slyA -1.1 -1.6 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0430 cyoC -1.1 -2.6 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b0630 lipB -1.1 -1.9 protein of lipoate biosynthesis (VIMSS) compare
b2652 ileY -1.1 -4.0 tRNA-Ile (NCBI) compare
b0737 tolQ -1.1 -2.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1730 ydjO -1.1 -2.3 predicted protein (RefSeq) compare
b1363 trkG -1.1 -3.6 Rac prophage; potassium transporter subunit (NCBI) compare
b0728 sucC -1.1 -3.7 succinyl-CoA synthetase subunit beta (NCBI) compare
b0722 sdhD -1.1 -2.9 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b2277 nuoM -1.1 -4.0 NADH dehydrogenase subunit M (NCBI) compare
b3726 pstA -1.1 -4.0 phosphate transporter subunit (NCBI) compare
b3204 ptsN -1.0 -3.5 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1130 phoP -1.0 -4.4 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b3035 tolC -1.0 -5.0 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1523 yneG -1.0 -1.7 hypothetical protein (NCBI) compare
b3811 xerC -1.0 -1.7 site-specific tyrosine recombinase XerC (NCBI) compare
b0723 sdhA -1.0 -5.5 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b3616 tdh -1.0 -3.7 L-threonine 3-dehydrogenase (NCBI) compare
b4202 rpsR -1.0 -1.5 30S ribosomal protein S18 (NCBI) compare
b3617 kbl -1.0 -4.5 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b4146 yjeK -1.0 -2.1 predicted lysine aminomutase (NCBI) compare
b0113 pdhR -1.0 -2.9 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b2822 recC -1.0 -3.3 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b2281 nuoI -1.0 -1.6 NADH dehydrogenase subunit I (NCBI) compare
b1144 ymfJ -1.0 -1.6 orf, hypothetical protein (VIMSS) compare
b4590 ybfK -1.0 -3.7 hypothetical protein (RefSeq) compare
b1323 tyrR -1.0 -3.0 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b1164 ycgZ -1.0 -1.9 hypothetical protein (NCBI) compare
b2340 sixA -1.0 -2.6 phosphohistidine phosphatase (NCBI) compare
b2363 yfdT -1.0 -2.1 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3652 recG -1.0 -5.3 ATP-dependent DNA helicase (NCBI) compare
b1286 rnb -1.0 -2.3 exoribonuclease II (NCBI) compare
b3728 pstS -0.9 -2.7 phosphate transporter subunit (NCBI) compare
b0429 cyoD -0.9 -2.6 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b4505 insX -0.9 -1.1 predicted IS protein (RefSeq) compare
b0767 ybhE -0.9 -2.5 6-phosphogluconolactonase (NCBI) compare
b1576 ydfD -0.9 -1.9 Qin prophage; predicted protein (NCBI) compare
b3500 gor -0.9 -1.0 glutathione reductase (NCBI) compare
b1409 ynbB -0.9 -3.9 predicted CDP-diglyceride synthase (NCBI) compare
b2279 nuoK -0.9 -1.7 NADH dehydrogenase subunit K (NCBI) compare
b0406 tgt -0.9 -4.0 queuine tRNA-ribosyltransferase (NCBI) compare
b3741 gidA -0.9 -4.0 glucose-inhibited division protein A (NCBI) compare
b1497 b1497 -0.9 -2.6 putative enzyme (VIMSS) compare
b4451 ryhB -0.9 -1.0 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b4685 yrbN -0.9 -1.9 expressed protein (RefSeq) compare
b2804 fucU -0.9 -1.9 L-fucose mutarotase (NCBI) compare
b4208 cycA -0.9 -4.8 D-alanine/D-serine/glycine transporter (NCBI) compare
b2284 nuoF -0.9 -2.6 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2990 hybG -0.9 -1.7 hydrogenase 2 accessory protein (NCBI) compare
b2330 yfcB -0.9 -1.5 putative adenine-specific methylase (VIMSS) compare
b1293 sapB -0.9 -1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2934 cmtB -0.9 -1.9 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b4180 rlmB -0.9 -3.8 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b2006 yeeW -0.8 -2.2 CP4-44 prophage; predicted protein (RefSeq) compare
b0729 sucD -0.8 -1.8 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1167 ymgC -0.8 -1.4 hypothetical protein (NCBI) compare
b2435 amiA -0.8 -2.9 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b2209 eco -0.8 -1.0 ecotin precursor (NCBI) compare
b3398 yrfF -0.8 -2.0 predicted inner membrane protein (NCBI) compare
b1259 yciG -0.8 -1.1 orf, hypothetical protein (VIMSS) compare
b2352 yfdI -0.8 -2.0 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b3649 rpoZ -0.8 -1.7 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3026 qseC -0.8 -2.8 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1095 fabF -0.8 -3.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0237 pepD -0.8 -4.0 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b1129 phoQ -0.8 -3.9 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b0720 gltA -0.8 -3.0 citrate synthase (NCBI) compare
b1058 yceO -0.8 -1.2 hypothetical protein (NCBI) compare
b4042 dgkA -0.8 -1.9 diacylglycerol kinase (NCBI) compare
b3791 rffA -0.8 -4.0 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2597 yfiA -0.8 -1.2 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1185 dsbB -0.8 -1.6 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0506 allR -0.8 -3.0 DNA-binding transcriptional repressor (NCBI) compare
b0001 thrL -0.8 -1.1 thr operon leader peptide (NCBI) compare
b0222 lpcA -0.8 -1.7 phosphoheptose isomerase (NCBI) compare
b0400 phoR -0.8 -4.3 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b2847 yqeI -0.8 -3.9 predicted transcriptional regulator (NCBI) compare
b3836 b3836 -0.8 -2.0 orf, hypothetical protein (VIMSS) compare
b1433 b1433 -0.8 -2.6 putative membrane transport protein (VIMSS) compare
b4651 yibW -0.8 -1.2 no description compare
b0542 renD -0.8 -1.5 orf, hypothetical protein (VIMSS) compare
b4497 yeeL -0.8 -3.2 no description compare
b4613 dinQ -0.8 -1.2 Damage inducible, function unknown (RefSeq) compare
b2276 nuoN -0.8 -2.8 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b1357 ydaS -0.8 -1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4593 ymgI -0.8 -1.5 hypothetical protein (RefSeq) compare
b4177 purA -0.8 -1.7 adenylosuccinate synthetase (NCBI) compare
b1351 racC -0.8 -2.0 Rac prophage; predicted protein (NCBI) compare
b1090 plsX -0.8 -2.6 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b2625 yfjI -0.7 -3.2 CP4-57 prophage; predicted protein (NCBI) compare
b1086 rluC -0.7 -2.9 23S rRNA pseudouridylate synthase (NCBI) compare
b3169 nusA -0.7 -1.4 transcription elongation factor NusA (NCBI) compare
b3483 yhhH -0.7 -1.3 orf, hypothetical protein (VIMSS) compare
b1125 potB -0.7 -2.0 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0724 sdhB -0.7 -2.7 succinate dehydrogenase, FeS subunit (NCBI) compare
b4660 yhiL -0.7 -1.7 no description compare
b1290 sapF -0.7 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4509 ylcG -0.7 -1.9 DLP12 prophage; predicted protein (NCBI) compare
b1946 fliN -0.7 -2.0 flagellar motor switch protein (NCBI) compare
b2897 ygfY -0.7 -1.4 hypothetical protein (NCBI) compare
b0548 ninE -0.7 -1.9 DLP12 prophage; conserved protein (NCBI) compare
b4410 ecnA -0.7 -0.8 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1536 ydeI -0.7 -2.7 hypothetical protein (NCBI) compare
b1462 b1462 -0.7 -2.1 orf, hypothetical protein (VIMSS) compare
b0432 cyoA -0.7 -4.1 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b2058 wcaB -0.7 -0.8 predicted acyl transferase (NCBI) compare
b0431 cyoB -0.7 -4.0 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b3807 cyaY -0.7 -2.8 frataxin-like protein (NCBI) compare
b2025 hisF -0.7 -1.8 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b1951 rcsA -0.7 -2.1 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b1548 nohA -0.7 -0.9 Qin prophage; predicted packaging protein (RefSeq) compare
b4546 ypeB -0.7 -0.9 hypothetical protein (NCBI) compare
b4253 yjgL -0.7 -3.2 orf, hypothetical protein (VIMSS) compare
b1008 ycdI -0.7 -1.7 hypothetical protein (NCBI) compare
b4191 ulaR -0.7 -2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0232 yafN -0.7 -0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0212 gloB -0.7 -1.4 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b2642 yfjW -0.7 -1.0 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3869 glnL -0.7 -3.2 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare
b1530 marR -0.7 -1.7 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3790 rffC -0.7 -2.5 TDP-fucosamine acetyltransferase (RefSeq) compare
b2498 upp -0.7 -1.7 uracil phosphoribosyltransferase (VIMSS) compare
b0279 yagM -0.7 -2.8 CP4-6 prophage; predicted protein (NCBI) compare
b1399 paaX -0.7 -1.5 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b1841 yobA -0.7 -0.9 hypothetical protein (NCBI) compare
b0920 ycbC -0.7 -1.9 conserved inner membrane protein (NCBI) compare
b4222 ytfP -0.7 -2.4 hypothetical protein (NCBI) compare
b0850 ybjC -0.6 -1.7 predicted inner membrane protein (NCBI) compare
b4533 ynfO -0.6 -1.2 Qin prophage; predicted protein (NCBI) compare
b4128 yjdK -0.6 -2.2 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Cholin acetate across organisms