Experiment set2IT079 for Pontibacter actiniarum KMM 6156, DSM 19842
marine broth with Carbenicillin disodium salt 0.0015 mg/ml
Group: stressMedia: marine_broth_2216 + Carbenicillin disodium salt (0.0015 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.5 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1735 C4
Specific Phenotypes
For 17 genes in this experiment
For stress Carbenicillin disodium salt in Pontibacter actiniarum KMM 6156, DSM 19842
For stress Carbenicillin disodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| ATP-dependent RNA helicases, bacterial | 1 |
| Catechol branch of beta-ketoadipate pathway | 1 |
| Muconate lactonizing enzyme family | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Benzoate degradation via hydroxylation
- Fluorobenzoate degradation
- Aminophosphonate metabolism
- High-mannose type N-glycan biosynthesis
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- Glycerophospholipid metabolism
- Sphingolipid metabolism
- Toluene and xylene degradation
- 1,4-Dichlorobenzene degradation
- Nicotinate and nicotinamide metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| catechol degradation to β-ketoadipate | 4 | 1 | 1 |
| catechol degradation III (ortho-cleavage pathway) | 6 | 2 | 1 |
| superpathway of salicylate degradation | 7 | 3 | 1 |
| aromatic compounds degradation via β-ketoadipate | 9 | 3 | 1 |
| mandelate degradation to acetyl-CoA | 18 | 2 | 1 |
| superpathway of aromatic compound degradation via 3-oxoadipate | 35 | 8 | 1 |