Experiment set2IT076 for Pontibacter actiniarum KMM 6156, DSM 19842
marine broth with Spectinomycin dihydrochloride pentahydrate 0.08 mg/ml
Group: stressMedia: marine_broth_2216 + Spectinomycin dihydrochloride pentahydrate (0.08 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.3 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1735 B6
Specific Phenotypes
For 17 genes in this experiment
For stress Spectinomycin dihydrochloride pentahydrate in Pontibacter actiniarum KMM 6156, DSM 19842
For stress Spectinomycin dihydrochloride pentahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cysteine Biosynthesis | 1 |
| Heat shock dnaK gene cluster extended | 1 |
| Multidrug Resistance Efflux Pumps | 1 |
| Thiamin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tryptophan metabolism
- Fatty acid metabolism
- Ubiquinone and menaquinone biosynthesis
- Lysine degradation
- Histidine metabolism
- Tyrosine metabolism
- Benzoxazinone biosynthesis
- Glutathione metabolism
- Naphthalene and anthracene degradation
- Benzoate degradation via CoA ligation
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| bacterial bioluminescence | 8 | 1 | 1 |