Experiment set2IT074 for Escherichia coli BW25113

LB with 5-Hydroxymethylfurfural 20 mM

Group: stress
Media: LB + 5-Hydroxymethylfurfural (20 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 962 C7,C8

Specific Phenotypes

For 1 genes in this experiment

For stress 5-Hydroxymethylfurfural in Escherichia coli BW25113

For stress 5-Hydroxymethylfurfural across organisms

SEED Subsystems

Subsystem	#Specific
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
putrescine biosynthesis I	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	1	1
putrescine biosynthesis II	3	1	1
superpathway of putrescine biosynthesis	4	3	1
spermidine biosynthesis III	4	2	1
superpathway of polyamine biosynthesis I	8	7	1
superpathway of polyamine biosynthesis II	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	11	1
superpathway of L-arginine and L-ornithine degradation	13	13	1
superpathway of arginine and polyamine biosynthesis	17	16	1