Experiment set2IT070 for Escherichia coli BW25113
LB with methylglyoxal 0.008 vol%
Group: stressMedia: LB + methylglyoxal (0.008 vol%)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 962 A7,A8
Specific Phenotypes
For 9 genes in this experiment
For stress methylglyoxal in Escherichia coli BW25113
For stress methylglyoxal across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA repair, bacterial RecFOR pathway | 2 |
| DNA-replication | 1 |
| Glycogen metabolism | 1 |
| Hfl operon | 1 |
| Maltose and Maltodextrin Utilization | 1 |
| Universal GTPases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Puromycin biosynthesis
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- alpha-Linolenic acid metabolism
- Trinitrotoluene degradation
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| polyphosphate metabolism | 2 | 2 | 1 |
| glycogen degradation I | 8 | 8 | 3 |
| glycogen degradation II | 6 | 4 | 2 |
| heme b biosynthesis I (aerobic) | 4 | 4 | 1 |
| heme b biosynthesis V (aerobic) | 4 | 4 | 1 |
| heme b biosynthesis II (oxygen-independent) | 4 | 4 | 1 |
| starch degradation V | 4 | 3 | 1 |
| starch degradation III | 4 | 2 | 1 |
| superpathway of heme b biosynthesis from uroporphyrinogen-III | 6 | 6 | 1 |
| ppGpp metabolism | 6 | 6 | 1 |
| superpathway of heme b biosynthesis from glycine | 8 | 7 | 1 |
| sucrose biosynthesis II | 8 | 6 | 1 |
| superpathway of heme b biosynthesis from glutamate | 10 | 10 | 1 |