Experiment set2IT068 for Agrobacterium fabrum C58
Gentibiose carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Gentibiose (5 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 10/20/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 4 genes in this experiment
For carbon source Gentibiose in Agrobacterium fabrum C58
For carbon source Gentibiose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| CytR regulation | 1 |
| Inositol catabolism | 1 |
| Pentose phosphate pathway | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Glutathione metabolism
- 1- and 2-Methylnaphthalene degradation
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pentose phosphate pathway (oxidative branch) I | 3 | 3 | 1 |
| pentose phosphate pathway | 8 | 8 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 1 |