Experiment set2IT062 for Pseudomonas fluorescens GW456-L13
Glycerol carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + Glycerol (20 mM), pH=7
Culturing: pseudo13_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/10/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 8 genes in this experiment
For carbon source Glycerol in Pseudomonas fluorescens GW456-L13
For carbon source Glycerol across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Lactate utilization | 4 |
| Murein hydrolase regulation and cell death | 2 |
| L-rhamnose utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyruvate fermentation to (S)-lactate | 1 | 1 | 1 |
| D-lactate to cytochrome bo oxidase electron transfer | 2 | 2 | 1 |
| methylglyoxal degradation I | 3 | 3 | 1 |
| 2-chloroacrylate degradation I | 3 | 2 | 1 |
| pyruvate fermentation to acetate and (S)-lactate I | 4 | 4 | 1 |
| pyruvate fermentation to acetate and lactate II | 4 | 3 | 1 |
| superpathway of methylglyoxal degradation | 8 | 5 | 1 |
| photorespiration I | 9 | 6 | 1 |
| homolactic fermentation | 12 | 10 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 6 | 1 |
| Bifidobacterium shunt | 15 | 13 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 1 |
| heterolactic fermentation | 18 | 15 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 15 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 15 | 1 |