Experiment set2IT056 for Escherichia coli BW25113

LB with Chloramphenicol 0.001 mg/ml

Group: stress
Media: LB + Chloramphenicol (0.001 mg/ml)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 959 A7,A8

Specific Phenotypes

For 12 genes in this experiment

For stress Chloramphenicol in Escherichia coli BW25113

For stress Chloramphenicol across organisms

SEED Subsystems

Subsystem	#Specific
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
Oxidative stress	1
Ribosome biogenesis bacterial	1
Transport of Zinc	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-methyl-5'-thioadenosine degradation II	1	1	1
adenine and adenosine salvage III	4	4	3
adenine and adenosine salvage V	3	3	2
purine ribonucleosides degradation	6	6	3
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
adenine and adenosine salvage I	2	2	1
pseudouridine degradation	2	2	1
adenosine nucleotides degradation II	5	5	2
polyisoprenoid biosynthesis (E. coli)	5	5	1
purine nucleotides degradation II (aerobic)	11	11	2
nucleoside and nucleotide degradation (archaea)	10	4	1
L-methionine salvage cycle III	11	5	1
superpathway of purine nucleotide salvage	14	13	1