Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| phosphatidylcholine biosynthesis V | 3 | 3 | 3 |
| L-glutamine biosynthesis I | 1 | 1 | 1 |
| L-aspartate biosynthesis | 1 | 1 | 1 |
| L-aspartate degradation I | 1 | 1 | 1 |
| L-asparagine degradation I | 1 | 1 | 1 |
| 3-(4-hydroxyphenyl)pyruvate biosynthesis | 1 | 1 | 1 |
| D-galactarate degradation II | 3 | 3 | 2 |
| D-glucarate degradation II | 3 | 3 | 2 |
| L-asparagine degradation III (mammalian) | 3 | 2 | 2 |
| L-glutamate degradation II | 2 | 2 | 1 |
| ammonia assimilation cycle I | 2 | 2 | 1 |
| choline degradation I | 2 | 2 | 1 |
| malate/L-aspartate shuttle pathway | 2 | 2 | 1 |
| D-galactose degradation II | 2 | 2 | 1 |
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| superpathway of L-aspartate and L-asparagine biosynthesis | 4 | 3 | 2 |
| ammonia assimilation cycle II | 2 | 1 | 1 |
| atromentin biosynthesis | 2 | 1 | 1 |
| arsenite to oxygen electron transfer | 2 | 1 | 1 |
| L-tryptophan degradation IV (via indole-3-lactate) | 2 | 1 | 1 |
| pyruvate fermentation to acetate VIII | 2 | 1 | 1 |
| L-tyrosine degradation II | 2 | 1 | 1 |
| D-glucuronate degradation II | 5 | 5 | 2 |
| D-galacturonate degradation II | 5 | 5 | 2 |
| phosphatidylcholine biosynthesis III | 5 | 2 | 2 |
| choline-O-sulfate degradation | 3 | 3 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| L-phenylalanine biosynthesis I | 3 | 3 | 1 |
| L-tyrosine biosynthesis I | 3 | 3 | 1 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| L-aspartate degradation III (anaerobic) | 3 | 2 | 1 |
| superpathway of ammonia assimilation (plants) | 3 | 2 | 1 |
| L-carnitine degradation II | 3 | 2 | 1 |
| L-aspartate degradation II (aerobic) | 3 | 2 | 1 |
| sulfolactate degradation III | 3 | 1 | 1 |
| L-phenylalanine degradation II (anaerobic) | 3 | 1 | 1 |
| arsenite to oxygen electron transfer (via azurin) | 3 | 1 | 1 |
| L-lyxonate degradation | 3 | 1 | 1 |
| L-tyrosine degradation IV (to 4-methylphenol) | 3 | 1 | 1 |
| indole-3-acetate biosynthesis VI (bacteria) | 3 | 1 | 1 |
| (R)-cysteate degradation | 3 | 1 | 1 |
| L-valine biosynthesis | 4 | 4 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 6 | 2 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| 1,2-dichloroethane degradation | 4 | 3 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 2 | 1 |
| L-phenylalanine degradation III | 4 | 2 | 1 |
| L-tyrosine degradation III | 4 | 2 | 1 |
| trans-4-hydroxy-L-proline degradation II | 4 | 2 | 1 |
| L-tryptophan degradation VIII (to tryptophol) | 4 | 1 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 9 | 3 |
| adipate degradation | 5 | 5 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| cytosolic NADPH production (yeast) | 5 | 4 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| acrylate degradation I | 5 | 3 | 1 |
| L-tyrosine degradation I | 5 | 3 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 1 |
| propanoyl-CoA degradation II | 5 | 3 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 3 | 1 |
| trans-4-hydroxy-L-proline degradation I | 5 | 3 | 1 |
| D-xylose degradation V | 5 | 2 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 2 | 1 |
| D-xylose degradation III | 5 | 2 | 1 |
| N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation | 5 | 2 | 1 |
| superpathway of plastoquinol biosynthesis | 5 | 2 | 1 |
| 4-hydroxybenzoate biosynthesis I (eukaryotes) | 5 | 1 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 1 | 1 |
| myo-inositol degradation II | 5 | 1 | 1 |
| L-tyrosine degradation V (reductive Stickland reaction) | 5 | 1 | 1 |
| L-tryptophan degradation XIII (reductive Stickland reaction) | 5 | 1 | 1 |
| phosphatidylcholine biosynthesis IV | 5 | 1 | 1 |
| L-phenylalanine degradation VI (reductive Stickland reaction) | 5 | 1 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 7 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| TCA cycle VIII (Chlamydia) | 6 | 6 | 1 |
| superpathway of L-threonine biosynthesis | 6 | 6 | 1 |
| β-alanine biosynthesis II | 6 | 3 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 1 |
| D-arabinose degradation III | 6 | 3 | 1 |
| L-isoleucine biosynthesis IV | 6 | 3 | 1 |
| L-arabinose degradation III | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 2 | 1 |
| superpathway of phosphatidylcholine biosynthesis | 12 | 2 | 2 |
| coenzyme M biosynthesis II | 6 | 1 | 1 |
| superpathway of sulfolactate degradation | 6 | 1 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 2 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| myo-inositol degradation I | 7 | 6 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 1 |
| C4 photosynthetic carbon assimilation cycle, PEPCK type | 14 | 8 | 2 |
| L-isoleucine biosynthesis III | 7 | 4 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 4 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 4 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| L-valine degradation I | 8 | 5 | 1 |
| L-isoleucine biosynthesis II | 8 | 5 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 2 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 2 |
| superpathway of L-methionine biosynthesis (transsulfuration) | 9 | 8 | 1 |
| valproate β-oxidation | 9 | 5 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 3 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 2 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| L-arginine biosynthesis II (acetyl cycle) | 10 | 10 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 7 | 1 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 6 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| rosmarinic acid biosynthesis I | 10 | 2 | 1 |
| superpathway of phenylethylamine degradation | 11 | 3 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 2 |
| (S)-reticuline biosynthesis I | 11 | 1 | 1 |
| oleate β-oxidation | 35 | 27 | 3 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 12 | 1 |
| indole-3-acetate biosynthesis II | 12 | 3 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 1 |
| superpathway of phospholipid biosynthesis II (plants) | 28 | 12 | 2 |
| superpathway of rosmarinic acid biosynthesis | 14 | 3 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 22 | 2 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 13 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 17 | 1 |
| superpathway of L-threonine metabolism | 18 | 11 | 1 |
| streptomycin biosynthesis | 18 | 3 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 16 | 2 |
| aspartate superpathway | 25 | 24 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| anaerobic aromatic compound degradation (Thauera aromatica) | 27 | 1 | 1 |
| superpathway of chorismate metabolism | 59 | 38 | 2 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 18 | 1 |