Experiment set2IT047 for Escherichia coli BW25113

LB with Aluminum chloride hydrate 5 mM

Group: stress
Media: LB + Aluminum chloride hydrate (5 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 961 B5,B6

Specific Phenotypes

For 25 genes in this experiment

For stress Aluminum chloride hydrate in Escherichia coli BW25113

For stress Aluminum chloride hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Campylobacter Iron Metabolism	1
Iron acquisition in Vibrio	1
LOS core oligosaccharide biosynthesis	1
Lipid A modifications	1
Maltose and Maltodextrin Utilization	1
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Protein degradation	1
Redox-dependent regulation of nucleus processes	1
Transport of Iron	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Pentose phosphate pathway
• Fatty acid biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Glutathione metabolism
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-methyl-5'-thioadenosine degradation II	1	1	1
adenine and adenosine salvage III	4	4	3
adenine and adenosine salvage V	3	3	2
purine ribonucleosides degradation	6	6	3
siroheme biosynthesis	4	4	2
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
adenine and adenosine salvage I	2	2	1
polyphosphate metabolism	2	2	1
glutathione degradation (DUG pathway)	2	2	1
adenosine nucleotides degradation II	5	5	2
factor 430 biosynthesis	7	3	2
polyisoprenoid biosynthesis (E. coli)	5	5	1
NAD salvage pathway V (PNC V cycle)	5	3	1
purine nucleotides degradation II (aerobic)	11	11	2
ppGpp metabolism	6	6	1
γ-glutamyl cycle	6	5	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
NAD salvage pathway I (PNC VI cycle)	7	5	1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
lipid A-core biosynthesis (E. coli K-12)	10	9	1
lipid A-core biosynthesis (Salmonella)	10	8	1
nucleoside and nucleotide degradation (archaea)	10	4	1
L-methionine salvage cycle III	11	5	1
NAD salvage (plants)	11	5	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of NAD biosynthesis in eukaryotes	14	7	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
superpathway of (Kdo)2-lipid A biosynthesis	17	17	1
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
superpathway of Kdo2-lipid A biosynthesis	25	24	1