Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
| Pathway | #Steps | #Present | #Specific |
|---|
| formate oxidation to CO2 | 1 | 1 | 1 |
| long-chain fatty acid activation | 1 | 1 | 1 |
| purine ribonucleosides degradation | 6 | 6 | 5 |
| purine deoxyribonucleosides degradation I | 4 | 4 | 3 |
| L-selenocysteine biosynthesis I (bacteria) | 3 | 3 | 2 |
| purine deoxyribonucleosides degradation II | 3 | 3 | 2 |
| glycogen degradation I | 8 | 8 | 5 |
| dTDP-β-L-rhamnose biosynthesis | 5 | 5 | 3 |
| superpathway of purine deoxyribonucleosides degradation | 7 | 7 | 4 |
| siroheme biosynthesis | 4 | 4 | 2 |
| adenine and adenosine salvage III | 4 | 4 | 2 |
| polyphosphate metabolism | 2 | 2 | 1 |
| pseudouridine degradation | 2 | 2 | 1 |
| formate to dimethyl sulfoxide electron transfer | 2 | 2 | 1 |
| guanine and guanosine salvage I | 2 | 2 | 1 |
| formate to trimethylamine N-oxide electron transfer | 2 | 2 | 1 |
| di-trans,poly-cis-undecaprenyl phosphate biosynthesis | 2 | 2 | 1 |
| L-threonine degradation II | 2 | 2 | 1 |
| xanthine and xanthosine salvage | 2 | 2 | 1 |
| adenine and adenosine salvage I | 2 | 2 | 1 |
| nitrate reduction III (dissimilatory) | 2 | 2 | 1 |
| starch degradation V | 4 | 3 | 2 |
| γ-linolenate biosynthesis II (animals) | 2 | 1 | 1 |
| cinnamoyl-CoA biosynthesis | 2 | 1 | 1 |
| linoleate biosynthesis II (animals) | 2 | 1 | 1 |
| pyrimidine deoxyribonucleosides salvage | 5 | 4 | 2 |
| dTDP-4-O-demethyl-β-L-noviose biosynthesis | 5 | 3 | 2 |
| superpathway of pyrimidine deoxyribonucleosides degradation | 6 | 6 | 2 |
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 3 | 1 |
| pyrimidine deoxyribonucleosides degradation | 3 | 3 | 1 |
| superpathway of guanine and guanosine salvage | 3 | 3 | 1 |
| cardiolipin biosynthesis I | 3 | 3 | 1 |
| 2-deoxy-α-D-ribose 1-phosphate degradation | 3 | 3 | 1 |
| cardiolipin biosynthesis II | 3 | 3 | 1 |
| L-aspartate degradation III (anaerobic) | 3 | 3 | 1 |
| adenine and adenosine salvage V | 3 | 3 | 1 |
| L-aspartate degradation II (aerobic) | 3 | 3 | 1 |
| glycogen degradation II | 6 | 4 | 2 |
| formate to nitrite electron transfer | 3 | 2 | 1 |
| dTDP-L-daunosamine biosynthesis | 6 | 3 | 2 |
| dTDP-sibirosamine biosynthesis | 6 | 3 | 2 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 2 |
| oleate biosynthesis I (plants) | 3 | 1 | 1 |
| alkane biosynthesis II | 3 | 1 | 1 |
| factor 430 biosynthesis | 7 | 3 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 11 | 3 |
| O-antigen building blocks biosynthesis (E. coli) | 11 | 11 | 3 |
| dTDP-N-acetylthomosamine biosynthesis | 4 | 4 | 1 |
| guanosine nucleotides degradation III | 4 | 4 | 1 |
| inosine 5'-phosphate degradation | 4 | 4 | 1 |
| sucrose biosynthesis II | 8 | 6 | 2 |
| cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas) | 4 | 3 | 1 |
| phytol degradation | 4 | 3 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 6 | 3 |
| dTDP-N-acetylviosamine biosynthesis | 4 | 2 | 1 |
| L-selenocysteine biosynthesis II (archaea and eukaryotes) | 4 | 2 | 1 |
| starch degradation III | 4 | 2 | 1 |
| oxalate degradation VI | 4 | 2 | 1 |
| dTDP-6-deoxy-α-D-allose biosynthesis | 4 | 2 | 1 |
| dTDP-β-D-fucofuranose biosynthesis | 4 | 2 | 1 |
| phosphatidylcholine acyl editing | 4 | 2 | 1 |
| long chain fatty acid ester synthesis (engineered) | 4 | 1 | 1 |
| GDP-D-glycero-α-D-manno-heptose biosynthesis | 4 | 1 | 1 |
| pinosylvin metabolism | 4 | 1 | 1 |
| wax esters biosynthesis II | 4 | 1 | 1 |
| superpathway of pyrimidine deoxyribonucleoside salvage | 9 | 8 | 2 |
| sporopollenin precursors biosynthesis | 18 | 5 | 4 |
| polyisoprenoid biosynthesis (E. coli) | 5 | 5 | 1 |
| pentose phosphate pathway (non-oxidative branch) I | 5 | 5 | 1 |
| ADP-L-glycero-β-D-manno-heptose biosynthesis | 5 | 5 | 1 |
| adenosine nucleotides degradation II | 5 | 5 | 1 |
| CMP-3-deoxy-D-manno-octulosonate biosynthesis | 5 | 5 | 1 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 8 | 2 |
| NAD salvage pathway II (PNC IV cycle) | 5 | 4 | 1 |
| Kdo transfer to lipid IVA (Chlamydia) | 5 | 3 | 1 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 2 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 2 |
| octane oxidation | 5 | 2 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis | 5 | 2 | 1 |
| dTDP-α-D-mycaminose biosynthesis | 5 | 2 | 1 |
| oxalate degradation III | 5 | 2 | 1 |
| dTDP-3-acetamido-α-D-fucose biosynthesis | 5 | 2 | 1 |
| molybdopterin biosynthesis | 6 | 6 | 1 |
| ppGpp metabolism | 6 | 6 | 1 |
| fatty acid salvage | 6 | 5 | 1 |
| stearate biosynthesis II (bacteria and plants) | 6 | 5 | 1 |
| stearate biosynthesis IV | 6 | 4 | 1 |
| nucleoside and nucleotide degradation (halobacteria) | 6 | 3 | 1 |
| dTDP-D-desosamine biosynthesis | 6 | 2 | 1 |
| dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis | 6 | 2 | 1 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 2 | 1 |
| fluoroacetate and fluorothreonine biosynthesis | 6 | 1 | 1 |
| stearate biosynthesis I (animals) | 6 | 1 | 1 |
| superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis | 19 | 7 | 3 |
| superpathway of cardiolipin biosynthesis (bacteria) | 13 | 11 | 2 |
| cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 2 | 2 |
| superpathway of purine nucleotide salvage | 14 | 13 | 2 |
| NAD salvage pathway I (PNC VI cycle) | 7 | 5 | 1 |
| ureide biosynthesis | 7 | 5 | 1 |
| CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis | 7 | 4 | 1 |
| dTDP-β-L-olivose biosynthesis | 7 | 3 | 1 |
| dTDP-β-L-digitoxose biosynthesis | 7 | 3 | 1 |
| ceramide degradation by α-oxidation | 7 | 2 | 1 |
| dTDP-β-L-mycarose biosynthesis | 7 | 2 | 1 |
| capsaicin biosynthesis | 7 | 1 | 1 |
| icosapentaenoate biosynthesis III (8-desaturase, mammals) | 7 | 1 | 1 |
| icosapentaenoate biosynthesis II (6-desaturase, mammals) | 7 | 1 | 1 |
| arachidonate biosynthesis III (6-desaturase, mammals) | 7 | 1 | 1 |
| cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 3 | 2 |
| pentose phosphate pathway | 8 | 8 | 1 |
| dTDP-β-L-4-epi-vancosamine biosynthesis | 8 | 3 | 1 |
| dTDP-β-L-megosamine biosynthesis | 8 | 3 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 3 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 2 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| formaldehyde assimilation II (assimilatory RuMP Cycle) | 9 | 7 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 4 | 1 |
| dTDP-α-D-forosamine biosynthesis | 9 | 3 | 1 |
| dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis | 9 | 3 | 1 |
| starch degradation II | 9 | 1 | 1 |
| superpathway of novobiocin biosynthesis | 19 | 4 | 2 |
| lipid A-core biosynthesis (E. coli K-12) | 10 | 9 | 1 |
| superpathway of enterobacterial common antigen biosynthesis | 10 | 9 | 1 |
| Rubisco shunt | 10 | 9 | 1 |
| lipid A-core biosynthesis (Salmonella) | 10 | 8 | 1 |
| peptidoglycan recycling II | 10 | 7 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| suberin monomers biosynthesis | 20 | 3 | 2 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 38 | 4 |
| NAD salvage (plants) | 11 | 5 | 1 |
| superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis | 33 | 14 | 3 |
| superpathway of phospholipid biosynthesis III (E. coli) | 12 | 12 | 1 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 12 | 1 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | 12 | 10 | 1 |
| superpathway of C1 compounds oxidation to CO2 | 12 | 4 | 1 |
| superpathway of Kdo2-lipid A biosynthesis | 25 | 24 | 2 |
| peptidoglycan recycling I | 14 | 14 | 1 |
| Bifidobacterium shunt | 15 | 13 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 11 | 1 |
| purine nucleobases degradation I (anaerobic) | 15 | 6 | 1 |
| salinosporamide A biosynthesis | 15 | 3 | 1 |
| palmitate biosynthesis II (type II fatty acid synthase) | 31 | 29 | 2 |
| cutin biosynthesis | 16 | 2 | 1 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 17 | 2 |
| superpathway of (Kdo)2-lipid A biosynthesis | 17 | 17 | 1 |
| superpathway of glucose and xylose degradation | 17 | 17 | 1 |
| arsenic detoxification (mammals) | 17 | 8 | 1 |
| superpathway of L-threonine metabolism | 18 | 16 | 1 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 16 | 2 |
| superpathway of erythromycin biosynthesis | 19 | 2 | 1 |
| superpathway of megalomicin A biosynthesis | 22 | 3 | 1 |
| superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis | 23 | 3 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 51 | 2 |
| palmitate biosynthesis III | 29 | 21 | 1 |
| oleate β-oxidation | 35 | 32 | 1 |