Experiment set2IT044 for Agrobacterium fabrum C58

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D-Glucosamine Hydrochloride carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + D-Glucosamine Hydrochloride (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 10/20/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 11 genes in this experiment

For carbon source D-Glucosamine Hydrochloride in Agrobacterium fabrum C58

For carbon source D-Glucosamine Hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
Xylose utilization 4
Chitin and N-acetylglucosamine utilization 2
Sialic Acid Metabolism 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 1
N-acetylglucosamine degradation II 3 2 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 3 2
N-acetylneuraminate and N-acetylmannosamine degradation I 4 1 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
CMP-N-acetylneuraminate biosynthesis I (eukaryotes) 5 1 1
UDP-N-acetyl-D-glucosamine biosynthesis II 6 4 1
UDP-N-acetyl-D-galactosamine biosynthesis III 6 2 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
chitin degradation I (archaea) 7 1 1
chitin derivatives degradation 8 2 1
photorespiration III 9 6 1
photorespiration I 9 6 1
chitin biosynthesis 9 5 1
photorespiration II 10 6 1
CMP-legionaminate biosynthesis I 10 2 1
O-antigen building blocks biosynthesis (E. coli) 11 11 1
superpathway of N-acetylneuraminate degradation 22 12 2
superpathway of CMP-sialic acids biosynthesis 15 1 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 6 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 1