Experiment set2IT043 for Escherichia coli BW25113

Compare to:

LB with Cisplatin 0.05 mg/ml

200 most important genes:

  gene name fitness t score description  
b0472 recR -5.5 -5.3 recombination protein RecR (NCBI) conserved
b1913 uvrC -4.9 -5.8 excinuclease ABC, subunit C; repair of UV damage to DNA (VIMSS) conserved
b2565 recO -4.5 -4.4 DNA repair protein RecO (NCBI) conserved
b3700 recF -4.2 -8.0 recombination protein F (NCBI) conserved
b0779 uvrB -4.2 -5.7 excinuclease ABC subunit B (NCBI) conserved
b2620 smpB -3.7 -2.5 SsrA-binding protein (NCBI) compare
b2531 iscR -3.6 -2.4 DNA-binding transcriptional repressor (NCBI) compare
b1848 yebG -3.6 -2.0 conserved protein regulated by LexA (NCBI) compare
b2567 rnc -3.6 -2.4 ribonuclease III (NCBI) compare
b0949 uup -3.6 -7.2 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1861 ruvA -3.5 -2.4 Holliday junction DNA helicase motor protein (NCBI) compare
b3813 uvrD -3.5 -14.0 DNA-dependent ATPase I and helicase II (NCBI) compare
b2501 ppk -3.5 -8.2 polyphosphate kinase (NCBI) compare
b4058 uvrA -3.4 -15.5 excinuclease ABC subunit A (NCBI) conserved
b2910 zapA -3.3 -6.5 protein that localizes to the cytokinetic ring (NCBI) compare
b1863 ruvC -3.2 -4.2 Holliday junction resolvase (NCBI) compare
b3169 nusA -3.1 -2.9 transcription elongation factor NusA (NCBI) compare
b4172 hfq -2.9 -2.0 RNA-binding protein Hfq (NCBI) compare
b3387 dam -2.9 -12.0 DNA adenine methylase (NCBI) compare
b1860 ruvB -2.8 -2.6 Holliday junction DNA helicase B (NCBI) compare
b3121 yhaC -2.7 -3.1 hypothetical protein (NCBI) compare
b4685 yrbN -2.7 -3.1 expressed protein (RefSeq) compare
b1175 minD -2.6 -5.8 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b2830 nudH -2.6 -1.7 dinucleoside polyphosphate hydrolase (NCBI) compare
b3463 ftsE -2.6 -3.0 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4175 hflC -2.5 -9.3 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b4174 hflK -2.4 -9.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2808 gcvA -2.4 -5.6 DNA-binding transcriptional dual regulator (NCBI) compare
b0145 dksA -2.4 -3.5 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1114 mfd -2.4 -7.1 transcription-repair coupling factor (NCBI) conserved
b3792 wzxE -2.3 -9.1 O-antigen translocase (NCBI) compare
b2616 recN -2.3 -8.8 recombination and repair protein (NCBI) conserved
b3725 pstB -2.3 -1.5 phosphate transporter subunit (NCBI) compare
b3933 ftsN -2.2 -10.7 essential cell division protein (NCBI) compare
b2699 recA -2.2 -1.5 recombinase A (NCBI) compare
b3728 pstS -2.2 -4.1 phosphate transporter subunit (NCBI) compare
b3613 yibP -2.2 -5.7 putative membrane protein (VIMSS) compare
b0053 surA -2.2 -3.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2330 yfcB -2.2 -2.8 putative adenine-specific methylase (VIMSS) compare
b3390 aroK -2.1 -2.4 shikimate kinase I (NCBI) compare
b0890 ftsK -2.1 -6.6 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b4239 treC -2.1 -5.0 trehalose-6-P hydrolase (NCBI) compare
b3652 recG -2.1 -9.0 ATP-dependent DNA helicase (NCBI) compare
b2820 recB -2.1 -1.4 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3778 rep -2.1 -8.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3839 tatC -2.1 -9.3 TatABCE protein translocation system subunit (NCBI) compare
b3462 ftsX -2.0 -4.6 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4375 prfC -2.0 -6.2 peptide chain release factor 3 (NCBI) compare
b0439 lon -1.9 -5.0 DNA-binding ATP-dependent protease La (NCBI) compare
b0705 ybfL -1.9 -4.1 putative receptor protein (VIMSS) compare
b3290 trkA -1.9 -3.5 potassium transporter peripheral membrane component (NCBI) compare
b1176 minC -1.9 -3.5 septum formation inhibitor (NCBI) compare
b4146 yjeK -1.9 -2.8 predicted lysine aminomutase (NCBI) compare
b3261 fis -1.8 -3.4 DNA-binding protein Fis (NCBI) compare
b4389 radA -1.8 -7.9 predicted repair protein (NCBI) conserved
b1576 ydfD -1.8 -3.2 Qin prophage; predicted protein (NCBI) compare
b4201 priB -1.8 -2.8 primosomal replication protein N (NCBI) compare
b3726 pstA -1.8 -6.2 phosphate transporter subunit (NCBI) compare
b3727 pstC -1.8 -5.6 phosphate transporter subunit (NCBI) compare
b2314 dedD -1.8 -3.5 putative lipoprotein (VIMSS) compare
b3341 rpsG -1.8 -1.6 30S ribosomal protein S7 (NCBI) compare
b3163 nlpI -1.8 -4.5 hypothetical protein (NCBI) compare
b2784 relA -1.7 -7.6 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b2819 recD -1.7 -6.2 exonuclease V (RecBCD complex), alpha chain (NCBI) compare
b0149 mrcB -1.7 -10.1 penicillin-binding protein 1b (NCBI) compare
b2184 yejH -1.7 -7.1 DNA damage response helicase (yejH or radD) (from data) conserved
b3706 trmE -1.7 -5.4 tRNA modification GTPase (NCBI) compare
b0737 tolQ -1.7 -3.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2822 recC -1.7 -4.9 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b3741 gidA -1.7 -6.3 glucose-inhibited division protein A (NCBI) compare
b2945 endA -1.6 -5.7 DNA-specific endonuclease I (NCBI) conserved
b3017 sufI -1.6 -9.1 repressor protein for FtsI (NCBI) compare
b3961 oxyR -1.6 -8.9 DNA-binding transcriptional dual regulator (NCBI) compare
b2617 smpA -1.6 -3.1 small membrane protein A (VIMSS) compare
b3764 yifE -1.6 -4.5 hypothetical protein (NCBI) compare
b1642 slyA -1.6 -2.0 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1257 yciE -1.6 -3.2 hypothetical protein (NCBI) compare
b0471 ybaB -1.6 -1.5 hypothetical protein (NCBI) compare
b2642 yfjW -1.5 -2.2 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1167 ymgC -1.5 -2.3 hypothetical protein (NCBI) compare
b0782 moaB -1.5 -2.0 molybdopterin biosynthesis protein B (NCBI) compare
b0015 dnaJ -1.5 -7.5 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1084 rne -1.5 -4.0 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0222 lpcA -1.5 -2.8 phosphoheptose isomerase (NCBI) compare
b2894 xerD -1.5 -1.6 site-specific tyrosine recombinase XerD (NCBI) compare
b4443 gcvB -1.5 -1.3 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b4460 araH -1.5 -2.7 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b0500 ybbD -1.5 -1.7 hypothetical protein (RefSeq) compare
b3068 ygjF -1.5 -2.7 G/U mismatch-specific DNA glycosylase (NCBI) compare
b0118 acnB -1.5 -2.6 aconitate hydratase (NCBI) compare
b3935 priA -1.5 -2.9 primosome assembly protein PriA (NCBI) compare
b0407 yajC -1.4 -3.0 preprotein translocase subunit YajC (NCBI) compare
b1545 pinQ -1.4 -1.4 Qin prophage; predicted site-specific recombinase (NCBI) compare
b4480 hdfR -1.4 -4.9 transcriptional regulator HdfR (NCBI) compare
b1040 csgD -1.4 -4.3 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b1129 phoQ -1.4 -6.0 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1007 b1007 -1.4 -1.2 orf, hypothetical protein (VIMSS) compare
b1184 umuC -1.4 -4.7 DNA polymerase V subunit UmuC (NCBI) conserved
b3644 yicC -1.4 -4.9 hypothetical protein (NCBI) compare
b2343 b2343 -1.4 -1.7 orf, hypothetical protein (VIMSS) compare
b1187 fadR -1.4 -2.9 fatty acid metabolism regulator (NCBI) compare
b1001 yccE -1.4 -1.8 hypothetical protein (NCBI) compare
b3791 rffA -1.3 -6.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3162 deaD -1.3 -3.9 inducible ATP-independent RNA helicase (VIMSS) compare
b2576 srmB -1.3 -3.1 ATP-dependent RNA helicase (NCBI) compare
b2110 yehC -1.3 -3.6 predicted periplasmic pilin chaperone (NCBI) compare
b1183 umuD -1.3 -1.6 DNA polymerase V, subunit D (NCBI) compare
b0948 ycbY -1.3 -4.6 predicted methyltransferase (NCBI) compare
b1085 b1085 -1.3 -1.3 orf, hypothetical protein (VIMSS) compare
b2431 b2431 -1.3 -5.4 orf, hypothetical protein (VIMSS) compare
b2698 recX -1.3 -1.9 RecA regulator RecX (NCBI) compare
b3636 rpmG -1.3 -1.3 50S ribosomal protein L33 (NCBI) compare
b4041 plsB -1.3 -1.8 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2946 yggJ -1.3 -4.1 orf, hypothetical protein (VIMSS) compare
b0726 sucA -1.3 -2.8 alpha-ketoglutarate decarboxylase (NCBI) compare
b4171 miaA -1.3 -4.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4177 purA -1.2 -2.4 adenylosuccinate synthetase (NCBI) compare
b0142 folK -1.2 -1.1 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2938 speA -1.2 -7.0 arginine decarboxylase (NCBI) compare
b2650 ypjC -1.2 -2.1 hypothetical protein (RefSeq) compare
b2181 yejG -1.2 -3.9 hypothetical protein (NCBI) compare
b0920 ycbC -1.2 -3.4 conserved inner membrane protein (NCBI) compare
b0082 mraW -1.2 -2.3 S-adenosyl-methyltransferase (NCBI) compare
b3183 obgE -1.2 -1.5 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0605 ahpC -1.2 -3.8 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1763 topB -1.2 -2.9 DNA topoisomerase III (NCBI) compare
b3863 polA -1.1 -1.0 DNA polymerase I (NCBI) compare
b1279 yciS -1.1 -1.3 conserved inner membrane protein (NCBI) compare
b0685 ybfE -1.1 -2.2 orf, hypothetical protein (VIMSS) compare
b2629 yfjM -1.1 -1.9 CP4-57 prophage; predicted protein (NCBI) compare
b3483 yhhH -1.1 -1.8 orf, hypothetical protein (VIMSS) compare
b2055 wcaE -1.1 -1.6 predicted glycosyl transferase (NCBI) compare
b1754 b1754 -1.1 -1.8 orf, hypothetical protein (VIMSS) compare
b0603 ybdO -1.1 -2.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b2809 b2809 -1.1 -2.1 orf, hypothetical protein (VIMSS) compare
b4426 isrA -1.1 -1.7 unknown RNA (NCBI) compare
b0946 ycbW -1.1 -3.3 orf, hypothetical protein (VIMSS) conserved
b2393 nupC -1.1 -4.1 nucleoside (except guanosine) transporter (NCBI) compare
b1536 ydeI -1.1 -3.8 hypothetical protein (NCBI) compare
b4451 ryhB -1.1 -1.1 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b4373 rimI -1.1 -1.8 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b4436 ryeC -1.1 -1.1 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1728 ydjM -1.1 -2.3 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1269 rluB -1.1 -2.4 23S rRNA pseudouridylate synthase (NCBI) compare
b4202 rpsR -1.1 -1.7 30S ribosomal protein S18 (NCBI) compare
b1305 pspB -1.1 -1.4 phage shock protein B (NCBI) compare
b2847 yqeI -1.0 -4.9 predicted transcriptional regulator (NCBI) compare
b1837 yebW -1.0 -1.6 orf, hypothetical protein (VIMSS) compare
b4590 ybfK -1.0 -3.5 hypothetical protein (RefSeq) compare
b1130 phoP -1.0 -4.6 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b1095 fabF -1.0 -4.5 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b4155 yjeA -1.0 -1.1 putative lysyl-tRNA synthetase (VIMSS) compare
b2569 lepA -1.0 -3.2 GTP-binding protein LepA (NCBI) compare
b4253 yjgL -1.0 -4.8 orf, hypothetical protein (VIMSS) compare
b2796 sdaC -1.0 -6.8 predicted serine transporter (NCBI) compare
b1259 yciG -1.0 -2.2 orf, hypothetical protein (VIMSS) compare
b2934 cmtB -1.0 -2.2 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b4497 yeeL -1.0 -3.6 no description compare
b0418 pgpA -1.0 -3.3 phosphatidylglycerophosphatase A (NCBI) compare
b0060 polB -1.0 -6.1 DNA polymerase II (NCBI) conserved
b3405 ompR -1.0 -3.4 osmolarity response regulator (NCBI) compare
b2011 sbcB -1.0 -4.5 exonuclease I (NCBI) compare
b1551 b1551 -1.0 -1.7 orf, hypothetical protein (VIMSS) compare
b1830 prc -1.0 -3.6 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0406 tgt -1.0 -4.4 queuine tRNA-ribosyltransferase (NCBI) compare
b3651 trmH -1.0 -2.9 tRNA (Guanosine-2'-O-)-methyltransferase (NCBI) compare
b2652 ileY -1.0 -3.3 tRNA-Ile (NCBI) compare
b0279 yagM -1.0 -3.9 CP4-6 prophage; predicted protein (NCBI) compare
b4410 ecnA -1.0 -1.0 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b0630 lipB -1.0 -1.7 protein of lipoate biosynthesis (VIMSS) compare
b1497 b1497 -0.9 -2.5 putative enzyme (VIMSS) compare
b1954 dsrA -0.9 -1.8 regulatory antisense RNA (NCBI) compare
b0606 ahpF -0.9 -3.9 alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS) compare
b2741 rpoS -0.9 -3.6 RNA polymerase sigma factor (NCBI) compare
b4438 cyaR -0.9 -1.5 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1074 flgC -0.9 -1.4 flagellar basal-body rod protein C (NCBI) compare
b0798 ybiA -0.9 -1.5 hypothetical protein (NCBI) compare
b1290 sapF -0.9 -1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1409 ynbB -0.9 -3.0 predicted CDP-diglyceride synthase (NCBI) compare
b4577 sgrS -0.9 -1.4 small antisense RNA (NCBI) compare
b2859 ygeO -0.9 -2.3 hypothetical protein (RefSeq) compare
b2893 dsbC -0.9 -3.0 protein disulfide isomerase II (NCBI) compare
b2937 speB -0.9 -4.3 agmatinase (NCBI) compare
b3811 xerC -0.9 -1.8 site-specific tyrosine recombinase XerC (NCBI) compare
b0113 pdhR -0.9 -2.8 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b2892 recJ -0.9 -5.0 ssDNA exonuclease, 5' --> 3'-specific (NCBI) conserved
b2853 ygeI -0.9 -2.2 hypothetical protein (NCBI) compare
b4552 yrhC -0.9 -1.5 no description compare
b0143 pcnB -0.9 -3.9 poly(A) polymerase I (VIMSS) compare
b1063 yceB -0.9 -1.8 predicted lipoprotein (NCBI) compare
b4702 mgtL -0.9 -2.1 regulatory leader peptide for mgtA (NCBI) compare
b2811 ygdK -0.9 -2.5 predicted Fe-S metabolism protein (NCBI) compare
b4234 yjgA -0.9 -2.0 hypothetical protein (NCBI) compare
b3082 ygjM -0.9 -1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b3838 tatB -0.9 -3.4 sec-independent translocase (NCBI) compare
b1907 tyrP -0.9 -3.7 tyrosine transporter (NCBI) compare
b3609 secB -0.9 -2.0 export protein SecB (NCBI) compare
b1058 yceO -0.9 -1.0 hypothetical protein (NCBI) compare
b1749 xthA -0.9 -2.5 exonuclease III (NCBI) compare
b1194 ycgR -0.9 -2.1 protein involved in flagellar function (NCBI) compare


Specific Phenotypes

For 14 genes in this experiment

For stress Cisplatin in Escherichia coli BW25113

For stress Cisplatin across organisms