Experiment set2IT043 for Escherichia coli BW25113

LB with Cisplatin 0.05 mg/ml

Group: stress
Media: LB + Cisplatin (0.05 mg/ml)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 960 E7,E8

Specific Phenotypes

For 14 genes in this experiment

For stress Cisplatin in Escherichia coli BW25113

For stress Cisplatin across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	4
DNA repair, UvrABC system	3
DNA repair, bacterial	2
DNA repair, bacterial RecFOR pathway	2
Nudix proteins (nucleoside triphosphate hydrolases)	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
heme b biosynthesis V (aerobic)	4	4	1
heme b biosynthesis I (aerobic)	4	4	1
heme b biosynthesis II (oxygen-independent)	4	4	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	6	1
superpathway of heme b biosynthesis from glycine	8	7	1
superpathway of heme b biosynthesis from glutamate	10	10	1