Experiment set2IT042 for Echinicola vietnamensis KMM 6221, DSM 17526
marine broth with Carbenicillin disodium salt 0.2 mg/ml
Group: stressMedia: marine_broth_2216 + Carbenicillin disodium salt (0.2 mg/ml)
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.5 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1722 C3
Specific Phenotypes
For 15 genes in this experiment
For stress Carbenicillin disodium salt in Echinicola vietnamensis KMM 6221, DSM 17526
For stress Carbenicillin disodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Beta-lactamase | 1 |
| Chitin and N-acetylglucosamine utilization | 1 |
| Folate Biosynthesis | 1 |
| N-Acetyl-Galactosamine and Galactosamine Utilization | 1 |
| Predicted carbohydrate hydrolases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Penicillin and cephalosporin biosynthesis
- beta-Lactam resistance
- Cyanoamino acid metabolism
- Starch and sucrose metabolism
- Other glycan degradation
- Nucleotide sugars metabolism
- Aminosugars metabolism
- Glycosaminoglycan degradation
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Phenylpropanoid biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| neolinustatin bioactivation | 3 | 2 | 2 |
| linustatin bioactivation | 4 | 2 | 2 |
| linamarin degradation | 2 | 1 | 1 |
| lotaustralin degradation | 2 | 1 | 1 |
| cellulose degradation II (fungi) | 3 | 2 | 1 |
| chitin degradation II (Vibrio) | 6 | 3 | 2 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| chitin degradation III (Serratia) | 7 | 3 | 1 |
| peptidoglycan recycling II | 10 | 3 | 1 |
| peptidoglycan recycling I | 14 | 8 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |