Experiment set2IT038 for Escherichia coli BW25113

LB with Cobalt chloride hexahydrate 0.32 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.32 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 960 B1,B2

Specific Phenotypes

For 12 genes in this experiment

For stress Cobalt chloride hexahydrate in Escherichia coli BW25113

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
ATP-dependent RNA helicases, bacterial	1
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
DNA repair, bacterial	1
Listeria phi-A118-like prophages	1
Polyamine Metabolism	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Urea cycle and metabolism of amino groups
• Puromycin biosynthesis
• Pyrimidine metabolism
• Arginine and proline metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• Naphthalene and anthracene degradation
• Trinitrotoluene degradation
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
pseudouridine degradation	2	2	1
putrescine biosynthesis I	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
putrescine biosynthesis II	3	1	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	1	1
superpathway of putrescine biosynthesis	4	3	1
spermidine biosynthesis III	4	2	1
superpathway of polyamine biosynthesis I	8	7	1
superpathway of polyamine biosynthesis II	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	11	1
superpathway of L-arginine and L-ornithine degradation	13	13	1
superpathway of arginine and polyamine biosynthesis	17	16	1