Experiment set2IT037 for Escherichia coli BW25113

Compare to:

LB with Nickel (II) chloride hexahydrate 1 mM

200 most important genes:

  gene name fitness t score description  
b2318 truA -3.5 -4.1 tRNA pseudouridine synthase A (NCBI) compare
b2007 yeeX -3.4 -3.9 conserved protein (RefSeq) compare
b0437 clpP -3.2 -7.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0149 mrcB -3.0 -11.2 penicillin-binding protein 1b (NCBI) compare
b2504 yfgG -2.7 -5.0 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3741 gidA -2.6 -4.7 glucose-inhibited division protein A (NCBI) compare
b3778 rep -2.6 -8.3 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2501 ppk -2.6 -7.9 polyphosphate kinase (NCBI) compare
b1130 phoP -2.6 -6.9 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b4702 mgtL -2.5 -3.7 regulatory leader peptide for mgtA (NCBI) compare
b1167 ymgC -2.5 -2.8 hypothetical protein (NCBI) compare
b3204 ptsN -2.5 -5.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3792 wzxE -2.4 -9.0 O-antigen translocase (NCBI) compare
b3164 pnp -2.4 -3.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4049 dusA -2.3 -8.0 tRNA-dihydrouridine synthase A (RefSeq) conserved
b0888 trxB -2.3 -2.7 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1129 phoQ -2.3 -7.6 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b0145 dksA -2.3 -3.1 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3725 pstB -2.3 -1.6 phosphate transporter subunit (NCBI) compare
b0438 clpX -2.2 -6.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0402 proY -2.2 -9.4 predicted cryptic proline transporter (NCBI) compare
b3644 yicC -2.2 -6.2 hypothetical protein (NCBI) compare
b1293 sapB -2.2 -2.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0741 pal -2.2 -3.8 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4242 mgtA -2.1 -14.3 magnesium transporter (NCBI) compare
b0143 pcnB -2.1 -6.6 poly(A) polymerase I (VIMSS) compare
b1192 ldcA -2.1 -4.4 L,D-carboxypeptidase A (NCBI) compare
b0726 sucA -2.1 -2.7 alpha-ketoglutarate decarboxylase (NCBI) compare
b2567 rnc -2.0 -1.9 ribonuclease III (NCBI) compare
b2297 pta -2.0 -1.3 phosphate acetyltransferase (NCBI) compare
b4480 hdfR -1.9 -5.9 transcriptional regulator HdfR (NCBI) compare
b1105 ycfM -1.9 -3.1 predicted outer membrane lipoprotein (NCBI) compare
b3764 yifE -1.9 -3.6 hypothetical protein (NCBI) compare
b3649 rpoZ -1.8 -3.1 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3839 tatC -1.8 -8.5 TatABCE protein translocation system subunit (NCBI) compare
b1286 rnb -1.8 -2.9 exoribonuclease II (NCBI) compare
b1085 b1085 -1.8 -2.5 orf, hypothetical protein (VIMSS) compare
b0142 folK -1.8 -1.5 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3871 bipA -1.8 -9.3 GTP-binding protein (NCBI) compare
b3706 trmE -1.8 -5.2 tRNA modification GTPase (NCBI) compare
b3405 ompR -1.8 -4.5 osmolarity response regulator (NCBI) compare
b0500 ybbD -1.7 -2.5 hypothetical protein (RefSeq) compare
b3121 yhaC -1.7 -2.5 hypothetical protein (NCBI) compare
b3205 yhbJ -1.7 -3.8 hypothetical protein (NCBI) compare
b1182 hlyE -1.7 -2.8 hemolysin E (VIMSS) compare
b3423 glpR -1.7 -7.4 DNA-binding transcriptional repressor (NCBI) compare
b1729 ydjN -1.7 -3.2 predicted transporter (NCBI) compare
b3740 gidB -1.6 -9.1 glucose-inhibited division protein B (NCBI) conserved
b3414 gntY -1.6 -5.7 predicted gluconate transport associated protein (NCBI) compare
b3005 exbD -1.6 -4.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2894 xerD -1.6 -1.5 site-specific tyrosine recombinase XerD (NCBI) compare
b1863 ruvC -1.6 -3.0 Holliday junction resolvase (NCBI) compare
b1842 holE -1.6 -1.5 DNA polymerase III, theta subunit (NCBI) compare
b1291 sapD -1.6 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2479 gcvR -1.5 -3.2 transcriptional regulation of gcv operon (VIMSS) compare
b1235 rssB -1.5 -5.0 response regulator of RpoS (NCBI) compare
b2814 metZ -1.5 -1.7 tRNA-Met (NCBI) compare
b1860 ruvB -1.5 -1.7 Holliday junction DNA helicase B (NCBI) compare
b3791 rffA -1.5 -6.8 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3780 rhlB -1.5 -8.7 ATP-dependent RNA helicase (NCBI) compare
b4234 yjgA -1.5 -3.3 hypothetical protein (NCBI) compare
b3755 yieP -1.5 -6.0 predicted transcriptional regulator (NCBI) compare
b1920 fliY -1.5 -2.2 cystine transporter subunit (NCBI) compare
b2937 speB -1.5 -5.7 agmatinase (NCBI) compare
b0890 ftsK -1.5 -5.4 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2820 recB -1.5 -2.1 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3728 pstS -1.5 -4.0 phosphate transporter subunit (NCBI) compare
b1728 ydjM -1.4 -2.7 predicted inner membrane protein regulated by LexA (RefSeq) compare
b3006 exbB -1.4 -5.7 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3183 obgE -1.4 -1.5 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3742 mioC -1.4 -4.1 flavodoxin (NCBI) compare
b4481 rffT -1.4 -5.4 4-alpha-L-fucosyltransferase (NCBI) compare
b2906 visC -1.4 -4.8 hypothetical protein (NCBI) compare
b4551 yheV -1.4 -2.6 hypothetical protein (NCBI) compare
b4201 priB -1.4 -1.6 primosomal replication protein N (NCBI) compare
b1162 ycgE -1.4 -4.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3229 sspA -1.4 -3.4 stringent starvation protein A (NCBI) compare
b0630 lipB -1.4 -1.9 protein of lipoate biosynthesis (VIMSS) compare
b1273 yciN -1.4 -1.1 hypothetical protein (NCBI) compare
b0471 ybaB -1.3 -2.7 hypothetical protein (NCBI) compare
b2938 speA -1.3 -7.3 arginine decarboxylase (NCBI) compare
b1936 intG -1.3 -2.2 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0200 gmhB -1.3 -1.6 hypothetical protein (NCBI) compare
b1123 potD -1.3 -4.5 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b0388 aroL -1.3 -4.2 shikimate kinase II (NCBI) compare
b3979 thrT -1.3 -1.1 tRNA-Thr (NCBI) compare
b3916 pfkA -1.3 -2.7 6-phosphofructokinase (NCBI) compare
b3483 yhhH -1.3 -2.0 orf, hypothetical protein (VIMSS) compare
b0585 fes -1.3 -4.4 enterobactin/ferric enterobactin esterase (NCBI) compare
b2106 yohM -1.3 -4.6 membrane protein conferring nickel and cobalt resistance (NCBI) conserved
b1394 paaG -1.3 -1.9 enoyl-CoA hydratase (NCBI) compare
b3290 trkA -1.3 -2.8 potassium transporter peripheral membrane component (NCBI) compare
b0738 tolR -1.3 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0688 pgm -1.3 -2.6 phosphoglucomutase (NCBI) compare
b1865 nudB -1.3 -1.0 dATP pyrophosphohydrolase (NCBI) compare
b0740 tolB -1.3 -3.1 translocation protein TolB precursor (NCBI) compare
b0721 sdhC -1.3 -3.5 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b4208 cycA -1.2 -6.4 D-alanine/D-serine/glycine transporter (NCBI) compare
b4589 ylcI -1.2 -1.8 hypothetical protein (RefSeq) compare
b2688 gshA -1.2 -5.2 glutamate--cysteine ligase (NCBI) compare
b1138 ymfE -1.2 -1.4 e14 prophage; predicted inner membrane protein (NCBI) compare
b1361 ydaW -1.2 -1.2 orf, hypothetical protein (VIMSS) compare
b3613 yibP -1.2 -4.4 putative membrane protein (VIMSS) compare
b1084 rne -1.2 -3.1 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0660 ybeZ -1.2 -2.6 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b3811 xerC -1.2 -1.9 site-specific tyrosine recombinase XerC (NCBI) compare
b2168 fruK -1.2 -1.1 1-phosphofructokinase (NCBI) compare
b2822 recC -1.2 -3.4 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b2804 fucU -1.2 -2.2 L-fucose mutarotase (NCBI) compare
b4592 yccB -1.2 -1.5 hypothetical protein (RefSeq) compare
b3198 kdsC -1.2 -1.8 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2635 ypjK -1.2 -2.2 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0737 tolQ -1.2 -2.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3931 hslU -1.2 -7.1 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1307 pspD -1.2 -1.6 peripheral inner membrane phage-shock protein (NCBI) compare
b0739 tolA -1.1 -2.9 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2699 recA -1.1 -1.6 recombinase A (NCBI) compare
b2946 yggJ -1.1 -3.4 orf, hypothetical protein (VIMSS) compare
b3398 yrfF -1.1 -2.7 predicted inner membrane protein (NCBI) compare
b2642 yfjW -1.1 -2.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2912 ygfA -1.1 -2.0 putative ligase (VIMSS) compare
b3781 trxA -1.1 -3.0 thioredoxin 1 (VIMSS) compare
b1576 ydfD -1.1 -2.1 Qin prophage; predicted protein (NCBI) compare
b3863 polA -1.1 -1.0 DNA polymerase I (NCBI) compare
b1992 cobS -1.1 -1.9 cobalamin synthase (NCBI) compare
b3169 nusA -1.1 -2.0 transcription elongation factor NusA (NCBI) compare
b0784 moaD -1.1 -2.2 molybdopterin synthase, small subunit (NCBI) compare
b2528 iscA -1.1 -1.3 iron-sulfur cluster assembly protein (NCBI) compare
b2576 srmB -1.1 -2.6 ATP-dependent RNA helicase (NCBI) compare
b0636 ybeA -1.1 -3.2 hypothetical protein (NCBI) compare
b1861 ruvA -1.0 -1.7 Holliday junction DNA helicase motor protein (NCBI) compare
b1493 gadB -1.0 -1.5 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b0590 fepD -1.0 -3.5 iron-enterobactin transporter subunit (NCBI) compare
b4180 rlmB -1.0 -4.4 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b4371 rsmC -1.0 -4.4 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1446 ydcY -1.0 -1.2 hypothetical protein (NCBI) compare
b3836 b3836 -1.0 -2.7 orf, hypothetical protein (VIMSS) compare
b3963 fabR -1.0 -5.9 DNA-binding transcriptional repressor (RefSeq) compare
b3932 hslV -1.0 -6.2 ATP-dependent protease peptidase subunit (NCBI) compare
b1144 ymfJ -1.0 -1.6 orf, hypothetical protein (VIMSS) compare
b0226 dinJ -1.0 -1.7 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b0589 fepG -1.0 -3.9 iron-enterobactin transporter subunit (NCBI) compare
b1126 potA -1.0 -3.5 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b1497 b1497 -1.0 -2.4 putative enzyme (VIMSS) compare
b4482 yigE -1.0 -3.1 hypothetical protein (NCBI) compare
b3463 ftsE -1.0 -3.1 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2569 lepA -1.0 -2.7 GTP-binding protein LepA (NCBI) compare
b3180 yhbY -1.0 -3.5 predicted RNA-binding protein (NCBI) compare
b0723 sdhA -1.0 -5.2 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b1676 pykF -0.9 -2.5 pyruvate kinase (NCBI) compare
b1343 dbpA -0.9 -2.8 ATP-dependent RNA helicase, specific for 23S rRNA (NCBI) compare
b3844 fre -0.9 -3.4 NAD(P)H-flavin reductase (NCBI) compare
b0418 pgpA -0.9 -3.1 phosphatidylglycerophosphatase A (NCBI) compare
b3120 yhaB -0.9 -2.9 orf, hypothetical protein (VIMSS) compare
b4045 yjbJ -0.9 -1.8 predicted stress response protein (NCBI) compare
b2330 yfcB -0.9 -1.5 putative adenine-specific methylase (VIMSS) compare
b4222 ytfP -0.9 -3.2 hypothetical protein (NCBI) compare
b3029 ygiN -0.9 -1.7 quinol monooxygenase (NCBI) compare
b3462 ftsX -0.9 -3.7 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0728 sucC -0.9 -3.1 succinyl-CoA synthetase subunit beta (NCBI) compare
b3838 tatB -0.9 -4.3 sec-independent translocase (NCBI) compare
b0949 uup -0.9 -4.1 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1409 ynbB -0.9 -3.7 predicted CDP-diglyceride synthase (NCBI) compare
b4171 miaA -0.9 -3.4 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1382 ynbE -0.9 -1.4 predicted lipoprotein (NCBI) compare
b1125 potB -0.9 -2.2 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3237 argR -0.9 -2.8 arginine repressor (NCBI) compare
b1370 insH-5 -0.9 -1.3 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b1918 yecS -0.9 -2.1 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2947 gshB -0.9 -2.7 glutathione synthetase (NCBI) compare
b1363 trkG -0.9 -3.0 Rac prophage; potassium transporter subunit (NCBI) compare
b1161 ycgX -0.9 -1.4 hypothetical protein (NCBI) compare
b0118 acnB -0.9 -2.1 aconitate hydratase (NCBI) compare
b0592 fepB -0.9 -4.3 iron-enterobactin transporter subunit (NCBI) compare
b1837 yebW -0.9 -1.6 orf, hypothetical protein (VIMSS) compare
b4533 ynfO -0.8 -1.4 Qin prophage; predicted protein (NCBI) compare
b0724 sdhB -0.8 -3.0 succinate dehydrogenase, FeS subunit (NCBI) compare
b2055 wcaE -0.8 -2.0 predicted glycosyl transferase (NCBI) compare
b0729 sucD -0.8 -1.6 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3652 recG -0.8 -5.1 ATP-dependent DNA helicase (NCBI) compare
b1295 ymjA -0.8 -1.3 hypothetical protein (NCBI) compare
b1252 tonB -0.8 -1.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1753 ynjA -0.8 -1.4 hypothetical protein (NCBI) compare
b0053 surA -0.8 -2.7 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1231 tyrT -0.8 -0.9 tRNA-Tyr (NCBI) compare
b2689 yqaA -0.8 -1.7 conserved inner membrane protein (NCBI) compare
b3404 envZ -0.8 -5.3 osmolarity sensor protein (NCBI) compare
b0588 fepC -0.8 -3.8 iron-enterobactin transporter subunit (NCBI) compare
b4502 yeiW -0.8 -2.3 hypothetical protein (NCBI) compare
b2683 ygaH -0.8 -2.5 predicted inner membrane protein (NCBI) compare
b1835 yebU -0.8 -1.9 putative nucleolar proteins (VIMSS) compare
b1063 yceB -0.8 -1.6 predicted lipoprotein (NCBI) compare
b0393 rdgC -0.8 -4.3 recombination associated protein (NCBI) compare
b2796 sdaC -0.8 -5.3 predicted serine transporter (NCBI) compare
b1058 yceO -0.8 -1.2 hypothetical protein (NCBI) compare
b2110 yehC -0.8 -2.4 predicted periplasmic pilin chaperone (NCBI) compare
b3442 yhhZ -0.8 -1.4 hypothetical protein (NCBI) compare
b2532 yfhQ -0.8 -2.1 predicted methyltransferase (NCBI) compare
b4497 yeeL -0.8 -3.1 no description compare
b1475 fdnH -0.8 -2.8 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare


Specific Phenotypes

For 14 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113

For stress Nickel (II) chloride hexahydrate across organisms