Experiment set2IT037 for Escherichia coli BW25113

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LB with Nickel (II) chloride hexahydrate 1 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (1 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/30/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 960 A3,A4

Specific Phenotypes

For 14 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
ATP-dependent RNA helicases, bacterial 1
Alanine biosynthesis 1
Branched-Chain Amino Acid Biosynthesis 1
Cobalt-zinc-cadmium resistance 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Heat shock dnaK gene cluster extended 1
Nudix proteins (nucleoside triphosphate hydrolases) 1
Proline, 4-hydroxyproline uptake and utilization 1
Pyruvate Alanine Serine Interconversions 1
Queuosine-Archaeosine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
thiosulfate disproportionation IV (rhodanese) 1 1 1
L-cysteine degradation III 2 2 1
L-alanine biosynthesis I 2 2 1
NAD salvage pathway III (to nicotinamide riboside) 3 3 1
queuosine biosynthesis I (de novo) 4 4 1
superpathway of L-alanine biosynthesis 4 4 1
NAD salvage pathway II (PNC IV cycle) 5 4 1
queuosine biosynthesis III (queuosine salvage) 5 3 1
sulfide oxidation IV (mitochondria) 5 2 1
NAD salvage pathway I (PNC VI cycle) 7 5 1
NAD salvage (plants) 11 5 1