Experiment set2IT033 for Escherichia coli BW25113

Compare to:

LB with Doxycycline hyclate 0.001 mg/ml

200 most important genes:

  gene name fitness t score description  
b0462 acrB -2.9 -8.9 multidrug efflux system protein (NCBI) compare
b0463 acrA -2.9 -5.8 multidrug efflux system (NCBI) compare
b3199 yrbK -2.9 -2.0 hypothetical protein (NCBI) compare
b1129 phoQ -2.8 -5.3 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1130 phoP -2.8 -4.2 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b3193 yrbD -2.6 -6.8 predicted ABC-type organic solvent transporter (NCBI) compare
b3035 tolC -2.6 -3.6 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3194 yrbE -2.6 -7.7 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3082 ygjM -2.5 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b2188 yejM -2.5 -4.9 predicted hydrolase, inner membrane (NCBI) compare
b2346 vacJ -2.4 -5.0 predicted lipoprotein (NCBI) compare
b1855 lpxM -2.4 -4.2 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0737 tolQ -2.4 -2.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3632 rfaQ -2.3 -11.6 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3195 yrbF -2.3 -6.9 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3465 yhhF -2.3 -4.0 predicted methyltransferase (NCBI) compare
b1677 lpp -2.3 -2.5 murein lipoprotein (NCBI) compare
b0957 ompA -2.2 -3.8 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3627 rfaI -2.2 -10.7 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3021 ygiT -2.2 -2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3191 yrbB -2.2 -5.7 orf, hypothetical protein (VIMSS) compare
b2017 yefM -2.1 -1.9 orf, hypothetical protein (VIMSS) compare
b3192 yrbC -2.1 -5.9 predicted ABC-type organic solvent transporter (NCBI) compare
b0178 hlpA -2.0 -2.6 periplasmic chaperone (NCBI) compare
b0877 ybjX -2.0 -5.3 hypothetical protein (NCBI) compare
b2571 rseB -2.0 -4.5 periplasmic negative regulator of sigmaE (NCBI) compare
b1651 gloA -2.0 -2.7 glyoxalase I, Ni-dependent (NCBI) compare
b4608 ryfC -1.9 -2.6 Novel sRNA, function unknown (RefSeq) compare
b4414 tff -1.9 -2.7 unknown RNA (NCBI) compare
b3163 nlpI -1.8 -2.9 hypothetical protein (NCBI) compare
b1830 prc -1.8 -3.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0658 ybeX -1.8 -5.8 predicteed ion transport (NCBI) compare
b4546 ypeB -1.8 -1.5 hypothetical protein (NCBI) compare
b1531 marA -1.8 -2.8 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b3631 rfaG -1.8 -8.7 glucosyltransferase I (NCBI) compare
b2174 b2174 -1.7 -5.4 orf, hypothetical protein (VIMSS) compare
b1413 hrpA -1.7 -5.4 ATP-dependent helicase (RefSeq) conserved
b1685 ydiH -1.7 -1.7 predicted protein (RefSeq) compare
b3784 rfe -1.7 -7.5 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b2617 smpA -1.7 -2.6 small membrane protein A (VIMSS) compare
b2055 wcaE -1.6 -3.1 predicted glycosyl transferase (NCBI) compare
b4172 hfq -1.6 -1.1 RNA-binding protein Hfq (NCBI) compare
b3636 rpmG -1.6 -1.0 50S ribosomal protein L33 (NCBI) compare
b3764 yifE -1.6 -3.2 hypothetical protein (NCBI) compare
b4370 leuQ -1.6 -1.7 tRNA-Leu (NCBI) compare
b4179 vacB -1.6 -4.4 putative enzyme (VIMSS) compare
b3625 rfaY -1.6 -7.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1380 ldhA -1.6 -1.6 D-lactate dehydrogenase (NCBI) compare
b1286 rnb -1.5 -1.4 exoribonuclease II (NCBI) compare
b4450 arcZ -1.5 -2.6 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b0145 dksA -1.5 -2.4 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b2064 asmA -1.5 -4.5 predicted assembly protein (NCBI) compare
b3628 rfaB -1.5 -8.3 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2572 rseA -1.4 -3.1 anti-sigma factor (NCBI) compare
b3121 yhaC -1.4 -1.8 hypothetical protein (NCBI) compare
b1104 ycfL -1.4 -1.9 hypothetical protein (NCBI) compare
b1183 umuD -1.4 -1.5 DNA polymerase V, subunit D (NCBI) compare
b0741 pal -1.4 -1.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1295 ymjA -1.4 -1.7 hypothetical protein (NCBI) compare
b0740 tolB -1.4 -2.0 translocation protein TolB precursor (NCBI) compare
b3787 rffD -1.4 -6.4 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0623 cspE -1.4 -1.7 cold shock protein E (NCBI) compare
b3341 rpsG -1.3 -1.4 30S ribosomal protein S7 (NCBI) compare
b0802 ybiJ -1.3 -1.9 hypothetical protein (NCBI) compare
b3842 rfaH -1.3 -2.3 transcriptional activator RfaH (NCBI) compare
b1684 sufA -1.3 -2.0 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b2807 ygdD -1.3 -2.6 conserved inner membrane protein (NCBI) compare
b2532 yfhQ -1.3 -2.3 predicted methyltransferase (NCBI) compare
b3649 rpoZ -1.3 -1.5 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3387 dam -1.3 -6.8 DNA adenine methylase (NCBI) compare
b1831 proQ -1.3 -2.6 putative solute/DNA competence effector (NCBI) compare
b3207 yrbL -1.3 -3.4 hypothetical protein (NCBI) compare
b0440 hupB -1.2 -3.1 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b1347 ydaC -1.2 -1.8 Rac prophage; predicted protein (NCBI) compare
b3961 oxyR -1.2 -6.1 DNA-binding transcriptional dual regulator (NCBI) compare
b0739 tolA -1.2 -1.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0912 ihfB -1.2 -2.1 integration host factor subunit beta (NCBI) compare
b0641 rlpB -1.2 -1.7 minor lipoprotein (NCBI) compare
b2634 yfjR -1.2 -2.1 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0738 tolR -1.2 -1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0222 lpcA -1.2 -1.1 phosphoheptose isomerase (NCBI) compare
b4550 yhdL -1.2 -3.0 hypothetical protein (NCBI) compare
b4176 yjeT -1.2 -2.3 conserved inner membrane protein (NCBI) compare
b4416 rybA -1.2 -1.6 unknown RNA (NCBI) compare
b2512 yfgL -1.2 -2.3 protein assembly complex, lipoprotein component (NCBI) compare
b3652 recG -1.1 -4.7 ATP-dependent DNA helicase (NCBI) compare
b3052 rfaE -1.1 -2.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1213 ychQ -1.1 -2.1 predicted transcriptional regulator (NCBI) compare
b3786 rffE -1.1 -6.3 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2558 mltF -1.1 -5.8 predicted periplasmic binding protein/transglycosylase (RefSeq) compare
b1448 yncA -1.1 -1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0880 cspD -1.1 -1.0 cold shock protein homolog (NCBI) compare
b0819 ybiS -1.1 -4.1 hypothetical protein (NCBI) compare
b1727 yniC -1.1 -1.6 predicted hydrolase (NCBI) compare
b0049 apaH -1.1 -2.9 diadenosinetetraphosphatase (NCBI) compare
b1085 b1085 -1.1 -1.1 orf, hypothetical protein (VIMSS) compare
b3630 rfaP -1.1 -4.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3785 wzzE -1.1 -6.1 putative transport protein (VIMSS) compare
b2494 yfgC -1.0 -3.8 predicted peptidase (NCBI) compare
b0759 galE -1.0 -2.9 UDP-galactose-4-epimerase (VIMSS) compare
b3026 qseC -1.0 -2.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0200 gmhB -1.0 -0.7 hypothetical protein (NCBI) compare
b1182 hlyE -1.0 -1.7 hemolysin E (VIMSS) compare
b3206 npr -1.0 -2.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4655 ythA -1.0 -1.3 expressed protein (RefSeq) compare
b1103 ycfF -1.0 -0.8 orf, hypothetical protein (VIMSS) compare
b1573 ydfC -1.0 -1.4 Qin prophage; predicted protein (RefSeq) compare
b1257 yciE -1.0 -1.8 hypothetical protein (NCBI) compare
b3229 sspA -1.0 -1.9 stringent starvation protein A (NCBI) compare
b1236 galU -1.0 -2.2 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4529 ydbJ -1.0 -0.9 hypothetical protein (NCBI) compare
b2829 ptsP -0.9 -4.8 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2831 mutH -0.9 -2.7 DNA mismatch repair protein (NCBI) compare
b1863 ruvC -0.9 -1.8 Holliday junction resolvase (NCBI) compare
b3609 secB -0.9 -2.3 export protein SecB (NCBI) compare
b3198 kdsC -0.9 -1.8 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b1992 cobS -0.9 -1.6 cobalamin synthase (NCBI) compare
b1160 elbA -0.9 -1.4 hypothetical protein (NCBI) compare
b2940 yqgC -0.9 -2.4 hypothetical protein (NCBI) compare
b0082 mraW -0.9 -1.6 S-adenosyl-methyltransferase (NCBI) compare
b2168 fruK -0.9 -1.3 1-phosphofructokinase (NCBI) compare
b1984 asnW -0.9 -0.8 tRNA-Asn (NCBI) compare
b0014 dnaK -0.9 -0.8 molecular chaperone DnaK (NCBI) compare
b1936 intG -0.9 -1.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4631 ykgQ -0.9 -1.1 no description compare
b1273 yciN -0.9 -0.8 hypothetical protein (NCBI) compare
b2846 yqeH -0.9 -1.8 orf, hypothetical protein (VIMSS) compare
b1848 yebG -0.9 -0.7 conserved protein regulated by LexA (NCBI) compare
b0605 ahpC -0.9 -2.2 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b4045 yjbJ -0.9 -1.5 predicted stress response protein (NCBI) compare
b0081 mraZ -0.9 -2.4 hypothetical protein (NCBI) compare
b3741 gidA -0.9 -3.0 glucose-inhibited division protein A (NCBI) compare
b2279 nuoK -0.9 -1.3 NADH dehydrogenase subunit K (NCBI) compare
b0053 surA -0.9 -1.0 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2317 dedA -0.9 -1.7 conserved inner membrane protein (NCBI) compare
b0405 queA -0.9 -2.9 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b3620 rfaF -0.9 -1.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3621 rfaC -0.8 -1.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b1258 yciF -0.8 -2.2 hypothetical protein (NCBI) compare
b1499 ydeO -0.8 -0.9 predicted DNA-binding transcriptional acfivator (NCBI) compare
b0471 ybaB -0.8 -1.5 hypothetical protein (NCBI) compare
b3101 yqjF -0.8 -1.2 orf, hypothetical protein (VIMSS) compare
b3417 malP -0.8 -4.7 maltodextrin phosphorylase (NCBI) compare
b3706 trmE -0.8 -2.7 tRNA modification GTPase (NCBI) compare
b3094 exuR -0.8 -2.4 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b2276 nuoN -0.8 -2.3 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b1609 rstB -0.8 -2.0 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b0862 artQ -0.8 -1.6 arginine transporter subunit (NCBI) compare
b1307 pspD -0.8 -1.0 peripheral inner membrane phage-shock protein (NCBI) compare
b2283 nuoG -0.8 -2.8 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b0436 tig -0.8 -3.3 trigger factor (NCBI) compare
b1919 yedO -0.8 -1.7 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b3811 xerC -0.8 -1.4 site-specific tyrosine recombinase XerC (NCBI) compare
b1643 ydhI -0.8 -2.4 predicted inner membrane protein (NCBI) compare
b0696 kdpC -0.8 -1.3 potassium-transporting ATPase subunit C (NCBI) compare
b1742 b1742 -0.8 -1.2 orf, hypothetical protein (VIMSS) compare
b2576 srmB -0.8 -1.4 ATP-dependent RNA helicase (NCBI) compare
b2469 narQ -0.8 -3.4 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b4202 rpsR -0.8 -0.7 30S ribosomal protein S18 (NCBI) compare
b0677 nagA -0.8 -2.6 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b1946 fliN -0.8 -1.7 flagellar motor switch protein (NCBI) compare
b3032 cpdA -0.8 -3.2 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b2217 rcsB -0.8 -2.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3237 argR -0.7 -2.0 arginine repressor (NCBI) compare
b1920 fliY -0.7 -1.1 cystine transporter subunit (NCBI) compare
b3293 yhdN -0.7 -2.3 hypothetical protein (NCBI) compare
b1434 ydcN -0.7 -0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3164 pnp -0.7 -1.2 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0249 ykfF -0.7 -1.2 CP4-6 prophage; predicted protein (NCBI) compare
b1271 yciK -0.7 -1.3 short chain dehydrogenase (NCBI) compare
b1278 pgpB -0.7 -1.0 phosphatidylglycerophosphatase B (NCBI) compare
b0232 yafN -0.7 -0.4 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1418 cybB -0.7 -1.5 cytochrome b(561) (VIMSS) compare
b1252 tonB -0.7 -1.3 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2362 yfdS -0.7 -2.1 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4396 rob -0.7 -3.5 DNA-binding transcriptional activator (NCBI) compare
b0144 yadB -0.7 -2.8 glutamyl-Q tRNA(Asp) synthetase (RefSeq) compare
b4592 yccB -0.7 -0.9 hypothetical protein (RefSeq) compare
b4402 yjjY -0.7 -0.9 hypothetical protein (NCBI) compare
b1486 ddpB -0.7 -1.4 D-ala-D-ala transporter subunit (NCBI) compare
b4178 yjeB -0.7 -2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1796 yoaG -0.7 -1.6 hypothetical protein (NCBI) compare
b4545 ypdJ -0.7 -0.9 no description compare
b1382 ynbE -0.7 -0.9 predicted lipoprotein (NCBI) compare
b4000 hupA -0.7 -1.6 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b4587 insN -0.7 -2.1 no description compare
b1728 ydjM -0.7 -1.4 predicted inner membrane protein regulated by LexA (RefSeq) compare
b3210 arcB -0.7 -3.0 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b1729 ydjN -0.7 -1.5 predicted transporter (NCBI) compare
b4627 ykfL -0.7 -1.3 no description compare
b3027 ygiZ -0.7 -1.4 conserved inner membrane protein (NCBI) compare
b1493 gadB -0.7 -0.9 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4375 prfC -0.7 -2.6 peptide chain release factor 3 (NCBI) compare
b3427 yzgL -0.7 -1.7 orf, hypothetical protein (VIMSS) compare
b1442 ydcU -0.7 -2.1 predicted spermidine/putrescine transporter subunit (NCBI) compare
b3463 ftsE -0.7 -1.3 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0613 citG -0.7 -2.0 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b1640 ydhH -0.7 -1.5 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b3039 ygiD -0.7 -2.3 predicted dioxygenase (RefSeq) compare
b3931 hslU -0.7 -3.9 ATP-dependent protease ATP-binding subunit (NCBI) compare


Specific Phenotypes

For 7 genes in this experiment

For stress Doxycycline hyclate in Escherichia coli BW25113

For stress Doxycycline hyclate across organisms