Experiment set2IT031 for Echinicola vietnamensis KMM 6221, DSM 17526

Compare to:

marine broth with D-Cycloserine 0.032 mg/ml

Group: stress
Media: marine_broth_2216 + D-Cycloserine (0.032 mg/ml)
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1725 C2

Specific Phenotypes

For 4 genes in this experiment

For stress D-Cycloserine in Echinicola vietnamensis KMM 6221, DSM 17526

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem #Specific
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Oxidative stress 1
Peptidoglycan Biosynthesis 1
Pyruvate Alanine Serine Interconversions 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) 8 8 1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) 8 7 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 11 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 1