Experiment set2IT023 for Pseudomonas sp. RS175
Malonic acid carbon source 10 mM
Group: carbon sourceMedia: MME_noCarbon + Malonic acid (10 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 1-Jul-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 11 genes in this experiment
For carbon source Malonic acid in Pseudomonas sp. RS175
For carbon source Malonic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Malonate decarboxylase | 8 |
| ZZ gjo need homes | 1 |
| cAMP signaling in bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- Biosynthesis of siderophore group nonribosomal peptides
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| malonate degradation I (biotin-independent) | 3 | 3 | 3 |
| malonate decarboxylase activation | 2 | 2 | 1 |
| fatty acid biosynthesis initiation (type II) | 3 | 3 | 1 |
| fatty acid biosynthesis initiation (mitochondria) | 4 | 2 | 1 |
| malonate degradation II (biotin-dependent) | 4 | 1 | 1 |
| fatty acid biosynthesis initiation (plant mitochondria) | 4 | 1 | 1 |
| superpathway of fatty acid biosynthesis initiation | 5 | 4 | 1 |
| pederin biosynthesis | 14 | 2 | 2 |
| bryostatin biosynthesis | 19 | 2 | 2 |
| mycobactin biosynthesis | 11 | 3 | 1 |
| superpathway of fatty acid biosynthesis I (E. coli) | 16 | 15 | 1 |
| mupirocin biosynthesis | 26 | 2 | 1 |
| corallopyronin A biosynthesis | 30 | 2 | 1 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 38 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 49 | 1 |