Experiment set2IT019 for Escherichia coli BW25113

LB with Sodium Chlorite 1.25 mM

Group: stress
Media: LB + Sodium Chlorite (1.25 mM)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 6/24/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 964 D3,D4

Specific Phenotypes

For 23 genes in this experiment

For stress Sodium Chlorite in Escherichia coli BW25113

For stress Sodium Chlorite across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	2
ATP-dependent RNA helicases, bacterial	1
Coenzyme A Biosynthesis	1
CytR regulation	1
Fermentations: Mixed acid	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Transport of Manganese	1
Trehalose Uptake and Utilization	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Purine metabolism
• Glycine, serine and threonine metabolism
• Cysteine metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
L-tryptophan degradation II (via pyruvate)	3	3	2
D-serine degradation	3	3	2
L-cysteine degradation II	3	2	2
nitrate reduction V (assimilatory)	2	1	1
alkylnitronates degradation	2	1	1
glycine betaine degradation III	7	4	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	4	3
glycine degradation	3	3	1
L-methionine biosynthesis II	6	5	2
L-methionine degradation II	3	2	1
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	3	2
nitrate reduction VII (denitrification)	5	2	1
nitrate reduction I (denitrification)	5	2	1
L-threonine degradation I	6	6	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
purine nucleobases degradation II (anaerobic)	24	17	3
superpathway of L-isoleucine biosynthesis I	13	13	1
hypoglycin biosynthesis	14	4	1
superpathway of branched chain amino acid biosynthesis	17	17	1
superpathway of L-threonine metabolism	18	16	1