Experiment set2IT014 for Agrobacterium fabrum C58

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D-Quinic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Quinic Acid (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 10/20/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 17 genes in this experiment

For carbon source Quinic Acid in Agrobacterium fabrum C58

For carbon source Quinic Acid across organisms

SEED Subsystems

Subsystem #Specific
Protocatechuate branch of beta-ketoadipate pathway 5
Catechol branch of beta-ketoadipate pathway 3
Chloroaromatic degradation pathway 3
Chorismate Synthesis 2
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 2
Biotin biosynthesis 1
DNA-binding regulatory proteins, strays 1
Quinate degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
3-oxoadipate degradation 2 2 2
long-chain fatty acid activation 1 1 1
protocatechuate degradation II (ortho-cleavage pathway) 4 4 3
aromatic compounds degradation via β-ketoadipate 9 8 5
catechol degradation III (ortho-cleavage pathway) 6 5 3
acetoacetate degradation (to acetyl CoA) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
toluene degradation III (aerobic) (via p-cresol) 11 7 5
superpathway of salicylate degradation 7 6 3
4-methylcatechol degradation (ortho cleavage) 7 3 3
chorismate biosynthesis from 3-dehydroquinate 5 5 2
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
5,6-dehydrokavain biosynthesis (engineered) 10 6 4
benzoyl-CoA biosynthesis 3 3 1
fatty acid salvage 6 5 2
ketolysis 3 2 1
gallate biosynthesis 3 2 1
polyhydroxybutanoate biosynthesis 3 2 1
quinate degradation I 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
plastoquinol-9 biosynthesis I 3 1 1
quinate degradation II 3 1 1
alkane biosynthesis II 3 1 1
oleate biosynthesis I (plants) 3 1 1
chorismate biosynthesis I 7 7 2
oleate β-oxidation 35 27 9
catechol degradation to β-ketoadipate 4 3 1
phytol degradation 4 3 1
4-sulfocatechol degradation 4 2 1
(2S)-ethylmalonyl-CoA biosynthesis 4 2 1
2-deoxy-D-ribose degradation II 8 2 2
long chain fatty acid ester synthesis (engineered) 4 1 1
wax esters biosynthesis II 4 1 1
phosphatidylcholine acyl editing 4 1 1
valproate β-oxidation 9 5 2
sporopollenin precursors biosynthesis 18 4 4
2-methyl-branched fatty acid β-oxidation 14 9 3
superpathway of L-tyrosine biosynthesis 10 10 2
superpathway of L-phenylalanine biosynthesis 10 10 2
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 5 1
glutaryl-CoA degradation 5 3 1
4-hydroxybenzoate biosynthesis III (plants) 5 3 1
fatty acid β-oxidation II (plant peroxisome) 5 3 1
L-tyrosine degradation I 5 3 1
ketogenesis 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
superpathway of plastoquinol biosynthesis 5 2 1
octane oxidation 5 2 1
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 2 2
ethylbenzene degradation (anaerobic) 5 1 1
fatty acid β-oxidation VII (yeast peroxisome) 5 1 1
pyruvate fermentation to acetone 5 1 1
isopropanol biosynthesis (engineered) 5 1 1
pyruvate fermentation to hexanol (engineered) 11 7 2
superpathway of aromatic compound degradation via 3-oxoadipate 35 15 6
stearate biosynthesis II (bacteria and plants) 6 5 1
chorismate biosynthesis II (archaea) 12 8 2
pyruvate fermentation to butanol II (engineered) 6 4 1
L-isoleucine degradation I 6 4 1
stearate biosynthesis IV 6 4 1
propanoate fermentation to 2-methylbutanoate 6 3 1
mandelate degradation to acetyl-CoA 18 8 3
superpathway of aerobic toluene degradation 30 12 5
4-ethylphenol degradation (anaerobic) 6 2 1
6-gingerol analog biosynthesis (engineered) 6 2 1
stearate biosynthesis I (animals) 6 1 1
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) 6 1 1
jasmonic acid biosynthesis 19 4 3
superpathway of L-tryptophan biosynthesis 13 13 2
fatty acid β-oxidation I (generic) 7 4 1
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 1
benzoyl-CoA degradation I (aerobic) 7 3 1
pyruvate fermentation to butanoate 7 3 1
ceramide degradation by α-oxidation 7 2 1
acetyl-CoA fermentation to butanoate 7 2 1
capsaicin biosynthesis 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
mevalonate pathway II (haloarchaea) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
mevalonate pathway I (eukaryotes and bacteria) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
vitamin E biosynthesis (tocopherols) 7 1 1
pyruvate fermentation to butanol I 8 4 1
2-methylpropene degradation 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
mevalonate pathway IV (archaea) 8 1 1
mevalonate pathway III (Thermoplasma) 8 1 1
isoprene biosynthesis II (engineered) 8 1 1
androstenedione degradation I (aerobic) 25 6 3
superpathway of aromatic amino acid biosynthesis 18 18 2
phenylacetate degradation I (aerobic) 9 3 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 3 1
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 3 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 2 1
4-oxopentanoate degradation 9 2 1
superpathway of testosterone and androsterone degradation 28 6 3
L-glutamate degradation V (via hydroxyglutarate) 10 4 1
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) 10 4 1
3-phenylpropanoate degradation 10 4 1
methyl tert-butyl ether degradation 10 3 1
L-lysine fermentation to acetate and butanoate 10 2 1
suberin monomers biosynthesis 20 3 2
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 4
superpathway of fatty acid biosynthesis II (plant) 43 38 4
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 6 1
ethylmalonyl-CoA pathway 11 3 1
superpathway of phenylethylamine degradation 11 3 1
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 8 4
L-glutamate degradation VII (to butanoate) 12 4 1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 1 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 5 1
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
androstenedione degradation II (anaerobic) 27 4 2
superpathway of glyoxylate cycle and fatty acid degradation 14 12 1
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
L-tryptophan degradation III (eukaryotic) 15 4 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
glycerol degradation to butanol 16 11 1
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 3 1
cutin biosynthesis 16 1 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 5 1
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 3 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 11 1
toluene degradation VI (anaerobic) 18 3 1
sitosterol degradation to androstenedione 18 1 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 2 1
superpathway of cholesterol degradation III (oxidase) 49 4 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
platensimycin biosynthesis 26 6 1
superpathway of ergosterol biosynthesis I 26 3 1
superpathway of fatty acids biosynthesis (E. coli) 53 49 2
1-butanol autotrophic biosynthesis (engineered) 27 18 1
palmitate biosynthesis III 29 28 1
superpathway of chorismate metabolism 59 38 2
superpathway of cholesterol biosynthesis 38 3 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 11 1
superpathway of L-lysine degradation 43 10 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 18 1