Experiment set2IT009 for Escherichia coli BW25113

Ammonium chloride nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + Ammonium chloride (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.9 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source Ammonium chloride in Escherichia coli BW25113

For nitrogen source Ammonium chloride across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• beta-Alanine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	2
phosphopantothenate biosynthesis III (archaea)	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
superpathway of coenzyme A biosynthesis II (plants)	10	5	2