Experiment set2IT009 for Agrobacterium fabrum C58

L-Rhamnose monohydrate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + L-Rhamnose monohydrate (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 10/20/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 9 genes in this experiment

For carbon source L-Rhamnose monohydrate in Agrobacterium fabrum C58

For carbon source L-Rhamnose monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
L-rhamnose utilization	6
DNA-replication	1
Homogentisate pathway of aromatic compound degradation	1
Lactate utilization	1
Respiratory dehydrogenases 1	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Galactose metabolism
• Purine metabolism
• Lipopolysaccharide biosynthesis
• Inositol phosphate metabolism
• Pyruvate metabolism
• Folate biosynthesis
• Biosynthesis of ansamycins

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
methylglyoxal degradation V	3	2	1
L-rhamnose degradation I	5	4	1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)	8	2	1
superpathway of fucose and rhamnose degradation	12	7	1