Experiment set2IT007 for Agrobacterium fabrum C58

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alpha-Lactose carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + alpha-Lactose (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 10/20/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 12 genes in this experiment

For carbon source alpha-Lactose in Agrobacterium fabrum C58

For carbon source alpha-Lactose across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 1
Galactosylceramide and Sulfatide metabolism 1
Inositol catabolism 1
Lactose and Galactose Uptake and Utilization 1
Lactose utilization 1
Maltose and Maltodextrin Utilization 1
Melibiose Utilization 1
NAD regulation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
lactose degradation III 1 1 1
neolinustatin bioactivation 3 2 2
D-galactose degradation II 2 2 1
linustatin bioactivation 4 2 2
lotaustralin degradation 2 1 1
linamarin degradation 2 1 1
xyloglucan degradation II (exoglucanase) 8 3 3
cellulose degradation II (fungi) 3 2 1
coumarin biosynthesis (via 2-coumarate) 5 2 1
α-tomatine degradation 6 1 1
firefly bioluminescence 14 2 1