Experiment set2IT002 for Pseudomonas sp. RS175

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L-Leucine carbon source 10 mM

Group: carbon source
Media: MME_noCarbon + L-Leucine (10 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 1-Jul-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 21 genes in this experiment

For carbon source L-Leucine in Pseudomonas sp. RS175

For carbon source L-Leucine across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 8
Glycine and Serine Utilization 2
Glycine cleavage system 2
Photorespiration (oxidative C2 cycle) 2
Arginine and Ornithine Degradation 1
Coenzyme A Biosynthesis 1
DNA Repair Base Excision 1
Folate Biosynthesis 1
Isobutyryl-CoA to Propionyl-CoA Module 1
L-rhamnose utilization 1
Proline, 4-hydroxyproline uptake and utilization 1
Respiratory dehydrogenases 1 1
Valine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-proline degradation I 3 3 2
glycine biosynthesis II 3 3 2
glycine cleavage 3 3 2
β-alanine degradation II 2 2 1
β-alanine degradation I 2 1 1
glycine degradation 3 3 1
dTMP de novo biosynthesis (mitochondrial) 3 3 1
tetrahydrofolate biosynthesis I 3 3 1
L-arginine degradation I (arginase pathway) 3 2 1
phosphopantothenate biosynthesis I 4 4 1
tetrahydromonapterin biosynthesis 4 3 1
phosphopantothenate biosynthesis III (archaea) 4 2 1
ethene biosynthesis II (microbes) 4 1 1
folate transformations III (E. coli) 9 9 2
propanoyl-CoA degradation II 5 3 1
folate transformations II (plants) 11 10 2
(5R)-carbapenem carboxylate biosynthesis 6 1 1
myo-inositol degradation I 7 6 1
L-Nδ-acetylornithine biosynthesis 7 5 1
2,4-dinitrotoluene degradation 7 1 1
L-citrulline biosynthesis 8 7 1
L-valine degradation I 8 5 1
superpathway of coenzyme A biosynthesis I (bacteria) 9 9 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
photorespiration III 9 5 1
photorespiration I 9 5 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
myo-, chiro- and scyllo-inositol degradation 10 6 1
photorespiration II 10 6 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of L-citrulline metabolism 12 9 1
folate transformations I 13 9 1
superpathway of chorismate metabolism 59 43 1