Experiment set2H24 for Kangiella aquimarina DSM 16071
marine broth with D-Cycloserine 0.03125 mg/ml
Group: stressMedia: marine_broth_2216 + D-Cycloserine (0.03125 mg/ml)
Culturing: Kang_ML4, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jake on 3/12/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 843 C1,C2
Specific Phenotypes
For 10 genes in this experiment
For stress D-Cycloserine in Kangiella aquimarina DSM 16071
For stress D-Cycloserine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Thioredoxin-disulfide reductase | 3 |
| DNA-binding regulatory proteins, strays | 1 |
| DNA repair, bacterial RecFOR pathway | 1 |
| Oxidative stress | 1 |
| Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Pyrimidine metabolism
- gamma-Hexachlorocyclohexane degradation
- Selenoamino acid metabolism
- Glutathione metabolism
- Toluene and xylene degradation
- Naphthalene and anthracene degradation
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| chlorinated phenols degradation | 4 | 4 | 4 |
| phenol degradation I (aerobic) | 1 | 1 | 1 |
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 2 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 6 | 3 |
| inosine 5'-phosphate degradation | 4 | 2 | 1 |
| adenosine nucleotides degradation I | 8 | 3 | 2 |
| purine nucleotides degradation I (plants) | 12 | 4 | 3 |
| guanosine nucleotides degradation III | 4 | 1 | 1 |
| guanosine nucleotides degradation I | 4 | 1 | 1 |
| guanosine nucleotides degradation II | 4 | 1 | 1 |
| adenosine nucleotides degradation II | 5 | 3 | 1 |
| superpathway of purines degradation in plants | 18 | 4 | 3 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 1 | 1 |
| ureide biosynthesis | 7 | 2 | 1 |
| tunicamycin biosynthesis | 9 | 1 | 1 |
| NAD salvage (plants) | 11 | 4 | 1 |