Experiment set29IT067 for Pseudomonas putida KT2440
D-Glucaric acid carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + D-Glucaric acid (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Matthias Schmidt on 4/13/21
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 4 genes in this experiment
For carbon source D-Glucaric acid in Pseudomonas putida KT2440
For carbon source D-Glucaric acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-galactarate, D-glucarate and D-glycerate catabolism | 3 |
| D-Galacturonate and D-Glucuronate Utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-glucarate degradation II | 3 | 3 | 2 |
| D-galactarate degradation II | 3 | 3 | 1 |
| D-glucarate degradation I | 4 | 3 | 1 |
| D-glucuronate degradation II | 5 | 4 | 1 |
| superpathway of D-glucarate and D-galactarate degradation | 5 | 4 | 1 |
| D-galacturonate degradation II | 5 | 3 | 1 |
| superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 13 | 2 |