Experiment set29IT057 for Pseudomonas putida KT2440
D-Galacturonic Acid monohydrate carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + D-Galacturonic Acid monohydrate (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Matthias Schmidt on 4/13/21
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 6 genes in this experiment
For carbon source D-Galacturonic Acid monohydrate in Pseudomonas putida KT2440
For carbon source D-Galacturonic Acid monohydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-Galacturonate and D-Glucuronate Utilization | 2 |
| D-galactarate, D-glucarate and D-glycerate catabolism | 2 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-glucuronate degradation III | 2 | 2 | 1 |
| D-galacturonate degradation III | 2 | 1 | 1 |
| D-glucuronate degradation II | 5 | 4 | 2 |
| D-galacturonate degradation II | 5 | 3 | 2 |
| D-galactarate degradation II | 3 | 3 | 1 |
| D-glucarate degradation II | 3 | 3 | 1 |
| superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 13 | 3 |