Experiment set29IT053 for Pseudomonas putida KT2440

Sodium gluconate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + D-Gluconic Acid sodium salt (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Matthias Schmidt on 4/13/21
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Gluconic Acid sodium salt in Pseudomonas putida KT2440

For carbon source D-Gluconic Acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
2-Ketogluconate Utilization	1
D-gluconate and ketogluconates metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.