Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| ethanol degradation II | 3 | 3 | 3 |
| L-glutamate biosynthesis I | 2 | 2 | 2 |
| L-glutamine degradation I | 1 | 1 | 1 |
| acetate conversion to acetyl-CoA | 1 | 1 | 1 |
| acetaldehyde biosynthesis I | 1 | 1 | 1 |
| acetate and ATP formation from acetyl-CoA III | 1 | 1 | 1 |
| glyphosate degradation II | 1 | 1 | 1 |
| L-glutamine degradation II | 1 | 1 | 1 |
| long-chain fatty acid activation | 1 | 1 | 1 |
| phytol degradation | 4 | 3 | 3 |
| ammonia assimilation cycle III | 3 | 3 | 2 |
| ethanol degradation IV | 3 | 3 | 2 |
| ethanol degradation III | 3 | 2 | 2 |
| putrescine degradation V | 2 | 2 | 1 |
| phenylethylamine degradation II | 2 | 2 | 1 |
| phenylethylamine degradation I | 2 | 2 | 1 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 3 |
| phospholipid remodeling (phosphatidate, yeast) | 2 | 1 | 1 |
| putrescine degradation I | 2 | 1 | 1 |
| ethylene glycol degradation | 2 | 1 | 1 |
| ethanol degradation I | 2 | 1 | 1 |
| linoleate biosynthesis II (animals) | 2 | 1 | 1 |
| phenylethanol degradation | 2 | 1 | 1 |
| pyruvate fermentation to ethanol II | 2 | 1 | 1 |
| γ-linolenate biosynthesis II (animals) | 2 | 1 | 1 |
| palmitoleate biosynthesis III (cyanobacteria) | 2 | 1 | 1 |
| octane oxidation | 5 | 4 | 2 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 4 |
| fatty acid salvage | 6 | 6 | 2 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| pyruvate decarboxylation to acetyl CoA I | 3 | 3 | 1 |
| oleate biosynthesis III (cyanobacteria) | 3 | 2 | 1 |
| putrescine degradation IV | 3 | 2 | 1 |
| hypotaurine degradation | 3 | 2 | 1 |
| L-leucine degradation III | 3 | 2 | 1 |
| L-isoleucine degradation II | 3 | 2 | 1 |
| L-phenylalanine degradation II (anaerobic) | 3 | 2 | 1 |
| L-isoleucine biosynthesis V | 3 | 2 | 1 |
| L-valine degradation II | 3 | 2 | 1 |
| pyruvate fermentation to ethanol III | 3 | 1 | 1 |
| pyruvate fermentation to ethanol I | 3 | 1 | 1 |
| oleate biosynthesis I (plants) | 3 | 1 | 1 |
| gentisate degradation I | 3 | 1 | 1 |
| alkane biosynthesis II | 3 | 1 | 1 |
| histamine degradation | 3 | 1 | 1 |
| L-methionine degradation III | 3 | 1 | 1 |
| styrene degradation | 3 | 1 | 1 |
| noradrenaline and adrenaline degradation | 13 | 8 | 4 |
| L-glutamate and L-glutamine biosynthesis | 7 | 6 | 2 |
| serotonin degradation | 7 | 4 | 2 |
| ceramide degradation by α-oxidation | 7 | 2 | 2 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| CDP-diacylglycerol biosynthesis I | 4 | 4 | 1 |
| CDP-diacylglycerol biosynthesis II | 4 | 4 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 3 | 1 |
| L-tryptophan degradation X (mammalian, via tryptamine) | 4 | 3 | 1 |
| putrescine degradation III | 4 | 3 | 1 |
| salidroside biosynthesis | 4 | 3 | 1 |
| chitin deacetylation | 4 | 2 | 1 |
| L-phenylalanine degradation III | 4 | 2 | 1 |
| fatty acid α-oxidation I (plants) | 4 | 2 | 1 |
| L-tyrosine degradation III | 4 | 2 | 1 |
| D-arabinose degradation II | 4 | 2 | 1 |
| butanol and isobutanol biosynthesis (engineered) | 8 | 3 | 2 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 4 |
| wax esters biosynthesis II | 4 | 1 | 1 |
| long chain fatty acid ester synthesis (engineered) | 4 | 1 | 1 |
| phosphatidylcholine acyl editing | 4 | 1 | 1 |
| 4-hydroxy-2-nonenal detoxification | 4 | 1 | 1 |
| cytidine-5'-diphosphate-glycerol biosynthesis | 4 | 1 | 1 |
| sporopollenin precursors biosynthesis | 18 | 4 | 4 |
| 2-methylcitrate cycle I | 5 | 5 | 1 |
| L-tyrosine degradation I | 5 | 5 | 1 |
| adipate degradation | 5 | 5 | 1 |
| ethanolamine utilization | 5 | 4 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 4 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| phenylethanol biosynthesis | 5 | 3 | 1 |
| phosphatidate biosynthesis (yeast) | 5 | 3 | 1 |
| CDP-diacylglycerol biosynthesis III | 5 | 3 | 1 |
| acetylene degradation (anaerobic) | 5 | 3 | 1 |
| pentachlorophenol degradation | 10 | 4 | 2 |
| (S)-propane-1,2-diol degradation | 5 | 2 | 1 |
| dopamine degradation | 5 | 2 | 1 |
| phosphatidylglycerol biosynthesis I | 6 | 6 | 1 |
| phosphatidylglycerol biosynthesis II | 6 | 6 | 1 |
| superpathway of phospholipid biosynthesis III (E. coli) | 12 | 10 | 2 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| 2-methylcitrate cycle II | 6 | 5 | 1 |
| stearate biosynthesis II (bacteria and plants) | 6 | 5 | 1 |
| stearate biosynthesis IV | 6 | 4 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 3 | 1 |
| 5-nitroanthranilate degradation | 6 | 2 | 1 |
| superpathway of stearidonate biosynthesis (cyanobacteria) | 6 | 2 | 1 |
| palmitoyl ethanolamide biosynthesis | 6 | 2 | 1 |
| superpathway of bitter acids biosynthesis | 18 | 3 | 3 |
| alkane oxidation | 6 | 1 | 1 |
| adlupulone and adhumulone biosynthesis | 6 | 1 | 1 |
| stearate biosynthesis I (animals) | 6 | 1 | 1 |
| lupulone and humulone biosynthesis | 6 | 1 | 1 |
| colupulone and cohumulone biosynthesis | 6 | 1 | 1 |
| 3-methylbutanol biosynthesis (engineered) | 7 | 6 | 1 |
| superpathway of glycol metabolism and degradation | 7 | 6 | 1 |
| thiazole component of thiamine diphosphate biosynthesis II | 7 | 4 | 1 |
| diacylglycerol and triacylglycerol biosynthesis | 7 | 3 | 1 |
| capsaicin biosynthesis | 7 | 3 | 1 |
| stigma estolide biosynthesis | 7 | 2 | 1 |
| icosapentaenoate biosynthesis III (8-desaturase, mammals) | 7 | 1 | 1 |
| limonene degradation IV (anaerobic) | 7 | 1 | 1 |
| arachidonate biosynthesis III (6-desaturase, mammals) | 7 | 1 | 1 |
| icosapentaenoate biosynthesis II (6-desaturase, mammals) | 7 | 1 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 7 | 1 |
| L-citrulline biosynthesis | 8 | 7 | 1 |
| superpathway of ornithine degradation | 8 | 6 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 4 | 1 |
| glutathione-mediated detoxification I | 8 | 3 | 1 |
| aromatic biogenic amine degradation (bacteria) | 8 | 3 | 1 |
| anandamide biosynthesis II | 8 | 2 | 1 |
| oleate β-oxidation | 35 | 30 | 4 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 6 | 1 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 5 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 5 | 1 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 4 | 1 |
| gliotoxin biosynthesis | 9 | 2 | 1 |
| cis-geranyl-CoA degradation | 9 | 2 | 1 |
| glutathione-mediated detoxification II | 9 | 1 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| suberin monomers biosynthesis | 20 | 4 | 2 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 38 | 4 |
| superpathway of phenylethylamine degradation | 11 | 11 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 9 | 1 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 8 | 1 |
| superpathway of L-citrulline metabolism | 12 | 9 | 1 |
| indole glucosinolate activation (intact plant cell) | 12 | 3 | 1 |
| anandamide biosynthesis I | 12 | 3 | 1 |
| camalexin biosynthesis | 12 | 2 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 11 | 1 |
| superpathway of cardiolipin biosynthesis (bacteria) | 13 | 9 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 4 | 1 |
| L-tryptophan degradation V (side chain pathway) | 13 | 1 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 10 | 1 |
| superpathway of phospholipid biosynthesis II (plants) | 28 | 10 | 2 |
| palmitate biosynthesis II (type II fatty acid synthase) | 31 | 29 | 2 |
| mixed acid fermentation | 16 | 12 | 1 |
| cutin biosynthesis | 16 | 1 | 1 |
| plasmalogen biosynthesis I (aerobic) | 16 | 1 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 6 | 1 |
| heterolactic fermentation | 18 | 12 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 14 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 13 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 12 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 51 | 2 |
| anaerobic aromatic compound degradation (Thauera aromatica) | 27 | 4 | 1 |
| palmitate biosynthesis III | 29 | 28 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 10 | 1 |