Experiment set28IT041 for Pseudomonas putida KT2440
L-pyroglutamic acid nitrogen source
Group: nitrogen sourceMedia: MOPS minimal media_Glucose_noNitrogen + L-pyroglutamic acid (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/16/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 5 genes in this experiment
For nitrogen source L-pyroglutamic acid in Pseudomonas putida KT2440
For nitrogen source L-pyroglutamic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cyanate hydrolysis | 1 |
| DNA-binding regulatory proteins, strays | 1 |
| Sialic Acid Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| urea degradation I | 3 | 2 | 2 |
| cyanuric acid degradation II | 5 | 3 | 2 |
| cyanuric acid degradation I | 5 | 2 | 2 |
| L-citrulline degradation | 3 | 3 | 1 |
| cyanate degradation | 3 | 2 | 1 |
| superpathway of allantoin degradation in yeast | 6 | 3 | 2 |
| L-arginine degradation V (arginine deiminase pathway) | 4 | 4 | 1 |
| superpathway of atrazine degradation | 8 | 3 | 2 |
| uracil degradation III | 5 | 3 | 1 |
| allantoin degradation IV (anaerobic) | 9 | 2 | 1 |