Experiment set28IT034 for Pseudomonas putida KT2440

Putrescine Dihydrochloride nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + Putrescine Dihydrochloride (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/16/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 3 genes in this experiment

For nitrogen source Putrescine Dihydrochloride in Pseudomonas putida KT2440

For nitrogen source Putrescine Dihydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Folate Biosynthesis	1
Tryptophan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Folate biosynthesis
• Nitrogen metabolism
• Biosynthesis of phenylpropanoids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine biosynthesis I	1	1	1
L-glutamine degradation I	1	1	1
ammonia assimilation cycle III	3	3	2
ammonia assimilation cycle II	2	2	1
ammonia assimilation cycle I	2	2	1
4-aminobenzoate biosynthesis I	2	2	1
L-glutamate biosynthesis I	2	2	1
putrescine degradation II	4	3	2
superpathway of ammonia assimilation (plants)	3	3	1
L-aspartate degradation III (anaerobic)	3	2	1
L-aspartate degradation II (aerobic)	3	2	1
L-glutamate and L-glutamine biosynthesis	7	6	2
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
superpathway of ornithine degradation	8	6	2
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	9	2
superpathway of L-arginine and L-ornithine degradation	13	11	2
L-citrulline biosynthesis	8	7	1
chloramphenicol biosynthesis	9	1	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
superpathway of candicidin biosynthesis	11	4	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
superpathway of L-citrulline metabolism	12	9	1
superpathway of chorismate metabolism	59	42	1