Experiment set28IT025 for Pseudomonas putida KT2440

3,5-Dinitrosalicylic acid nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + 3,5-Dinitrosalicylic acid (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/16/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 3 genes in this experiment

For nitrogen source 3,5-Dinitrosalicylic acid in Pseudomonas putida KT2440

For nitrogen source 3,5-Dinitrosalicylic acid across organisms

SEED Subsystems

Subsystem	#Specific
Lysine degradation	1
Orphan regulatory proteins	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.